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06-519 Anti-phospho-CREB (Ser133) Antibody

06-519
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, IH(P), IP, ChIP, Gel ElectrophoresisRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-519
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CREB (Ser133) Antibody
      Alternate Names
      • active transcription factor CREB
      • cAMP responsive element binding protein 1
      • cAMP-response element-binding protein-1
      • transactivator protein
      Background InformationCREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      • Gel Electrophoresis
      Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected phospho CREB (Ser133) in rat cerebral cortex, human cerebral cortex, and rat hippocampus tissue.
      Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated phospho CREB (Ser133) in 500 µg of BALB/3T3 A31+Forskolin cell lysate.
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho CREB (Ser133) in 4 µg of HEK293T cell lysate.
      Gel Shift (EMSA): This antibody has been shown to gel shift by independent labs.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the KID of human phospho CREB (Ser133).
      Epitopephospho-Ser133
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteMouse, Human, Rat. Predicted to react with Bovine, Canine, Porcine, Xenopus, Zebrafish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CREB1
      • CREB
      • MGC9284
      Modifications
      • Phosphorylation
      Purification MethodPurified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
      UniProt Number
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Forskolin treated BALB/3T3 clone A31 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho CREB (Ser133) in 10 µg of Forskolin treated BALB/3T3 clone A31 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-CREB (Ser133) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-CREB (Ser133) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho CREB (Ser133) - 2491214 2491214
      Anti-phospho CREB (Ser133) - 3018773 3018773
      Anti-phospho CREB (Ser133) - 3141397 3141397
      Anti-phospho CREB (Ser133) - 3172455 3172455
      Anti-phospho CREB (Ser133) - 3226541 3226541
      Anti-phospho CREB (Ser133) - 3237505 3237505
      Anti-phospho CREB (Ser133) - 3318328 3318328
      Anti-phospho CREB (Ser133) - 3460466 3460466
      Anti-phospho CREB (Ser133) - 3560005 3560005
      Anti-phospho CREB (Ser133) - 3671737 3671737

      References

      Reference overviewApplicationSpeciesPub Med ID
      Region and context-specific intracellular responses associated with cocaine-induced conditioned place preference expression.
      Nygard, SK; Klambatsen, A; Balouch, B; Quinones-Jenab, V; Jenab, S
      Neuroscience  287  1-8  2015

      Show Abstract
      25522720 25522720
      Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area.
      Koo, JW; Mazei-Robison, MS; LaPlant, Q; Egervari, G; Braunscheidel, KM; Adank, DN; Ferguson, D; Feng, J; Sun, H; Scobie, KN; Damez-Werno, DM; Ribeiro, E; Peña, CJ; Walker, D; Bagot, RC; Cahill, ME; Anderson, SA; Labonté, B; Hodes, GE; Browne, H; Chadwick, B; Robison, AJ; Vialou, VF; Dias, C; Lorsch, Z; Mouzon, E; Lobo, MK; Dietz, DM; Russo, SJ; Neve, RL; Hurd, YL; Nestler, EJ
      Nature neuroscience  18  415-22  2015

      Show Abstract
      25643298 25643298
      Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease.
      D'Souza, Y; Elharram, A; Soon-Shiong, R; Andrew, RD; Bennett, BM
      Molecular brain  8  27  2015

      Show Abstract
      25910195 25910195
      Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice.
      Villeda, SA; Plambeck, KE; Middeldorp, J; Castellano, JM; Mosher, KI; Luo, J; Smith, LK; Bieri, G; Lin, K; Berdnik, D; Wabl, R; Udeochu, J; Wheatley, EG; Zou, B; Simmons, DA; Xie, XS; Longo, FM; Wyss-Coray, T
      Nature medicine  20  659-63  2014

      Show Abstract
      ImmunohistochemistryMouse24793238 24793238
      Inhibition of phosphodiesterase10A attenuates morphine-induced conditioned place preference.
      Mu, Y; Ren, Z; Jia, J; Gao, B; Zheng, L; Wang, G; Friedman, E; Zhen, X
      Molecular brain  7  70  2014

      Show Abstract
      Immunofluorescence25252626 25252626
      M1 muscarinic receptor activation mediates cell death in M1-HEK293 cells.
      Graham, ES; Woo, KK; Aalderink, M; Fry, S; Greenwood, JM; Glass, M; Dragunow, M
      PloS one  8  e72011  2013

      Show Abstract
      24023725 24023725
      Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification.
      Regard, JB; Malhotra, D; Gvozdenovic-Jeremic, J; Josey, M; Chen, M; Weinstein, LS; Lu, J; Shore, EM; Kaplan, FS; Yang, Y
      Nature medicine  19  1505-12  2013

      Show Abstract
      Western Blotting24076664 24076664
      Sexually dimorphic intracellular responses after cocaine-induced conditioned place preference expression.
      Nygard, SK; Klambatsen, A; Hazim, R; Eltareb, MH; Blank, JC; Chang, AJ; Quinones-Jenab, V; Jenab, S
      Brain research  1520  121-33  2013

      Show Abstract
      23665060 23665060
      AAD-2004 Attenuates Progressive Neuronal Loss in the Brain of Tg-betaCTF99/B6 Mouse Model of Alzheimer Disease.
      Baek, IS; Kim, TK; Seo, JS; Lee, KW; Lee, YA; Cho, J; Gwag, BJ; Han, PL
      Experimental neurobiology  22  31-7  2013

      Show Abstract
      23585720 23585720
      A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis.
      Megill, A; Lee, T; DiBattista, AM; Song, JM; Spitzer, MH; Rubinshtein, M; Habib, LK; Capule, CC; Mayer, M; Turner, RS; Kirkwood, A; Yang, J; Pak, DT; Lee, HK; Hoe, HS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  9306-18  2013

      Show Abstract
      23719799 23719799

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