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OP104L Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

OP104L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M M Monoclonal Antibody

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OP104L-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
      Catalogue NumberOP104L
      Brand Family Calbiochem®
      References
      ReferencesLevine, A.J. 1997. Cell 88, 323.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      Cho, Y., et al. 1994. Science 265, 346.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlSK-OV-3 cells
      Positive controlA431 or SK-BR-3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
      Key Applications Not Immunofluorescence
      Not Paraffin Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
      Immunoprecipitation (1 µg/reaction, see comments, see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human p53 protein
      ImmunogenHuman
      Epitopewithin amino acids 306-393 of human p53
      ClonePAb1802
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) Certificates of Analysis

      TitleLot Number
      OP104L

      References

      Reference overview
      Levine, A.J. 1997. Cell 88, 323.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      Cho, Y., et al. 1994. Science 265, 346.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
      Immunoprecipitation (1 µg/reaction, see comments, see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
      Backgroundp53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human p53 protein
      Epitopewithin amino acids 306-393 of human p53
      ClonePAb1802
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlA431 or SK-BR-3 cells
      Negative controlSK-OV-3 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
      Toxicity Standard Handling
      ReferencesLevine, A.J. 1997. Cell 88, 323.
      Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
      Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
      Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
      Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
      Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
      Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
      Waldman, T., et al. 1995. Cancer Res. 55, 5187.
      Clarke, A.R., et al. 1994. Oncogene 9, 1767.
      Cho, Y., et al. 1994. Science 265, 346.
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Lane, D.P. 1992. Nature 358, 15.
      Application referencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.