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OP03 Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

OP03
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, Mk, M, Rb, RMMonoclonal Antibody

Pricing & Availability

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OP03-100UG
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      Glass bottle 100 μg
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      OP03-20UG
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          Glass bottle 20 μg
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          Description
          OverviewRecognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
          Catalogue NumberOP03
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlSK-OV-3 cells or normal skin
          Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. (within amino acids 371-380) Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Flow Cytometry
          Frozen Sections
          Gel Shift
          Application NotesFrozen Sections (10 µg/ml, see application references)
          Gel Shift (see comments)
          Immunoblotting (10 µg/ml, see application references)
          Immunofluorescence (1-20 µg/ml, see application references)
          Flow Cytometry (1-20 µg/ml)
          Immunoprecipitation (1 μg per sample, see application references)
          Application CommentsOriginally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpartially purified mouse p53
          ImmunogenMouse
          Epitopewithin amino acids 376-378 of human p53
          ClonePAb421
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Monkey
          • Mouse
          • Rabbit
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Certificates of Analysis

          TitleLot Number
          OP03

          References

          Reference overview
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.

          Brochure

          Title
          Interactive Pathway Calendar
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-July-2008 JSW
          ApplicationFrozen Sections (10 µg/ml, see application references)
          Gel Shift (see comments)
          Immunoblotting (10 µg/ml, see application references)
          Immunofluorescence (1-20 µg/ml, see application references)
          Flow Cytometry (1-20 µg/ml)
          Immunoprecipitation (1 μg per sample, see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
          HostMouse
          Immunogen speciesMouse
          Immunogenpartially purified mouse p53
          Epitopewithin amino acids 376-378 of human p53
          ClonePAb421
          IsotypeIgG2a
          Specieshuman, monkey, mouse, rabbit, rat
          Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
          Negative controlSK-OV-3 cells or normal skin
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsOriginally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.
          Application referencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. (within amino acids 371-380) Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies