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AB2201 Anti-mPER1 (residues 6-21) Antibody

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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWBRbSerumPolyclonal Antibody
Catalogue NumberAB2201
DescriptionAnti-mPER1 (residues 6-21) Antibody
Alternate Names
  • Circadian clock protein PERIOD 1
  • Circadian pacemaker protein Rigui
  • Period, drosophila
  • homolog of period 1
  • period (Drosophila) homolog 1
  • period homolog 1 (Drosophila)
Background InformationThis gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.
Product Information
  • Human brain lysate
PresentationSerum with 0.05% NaN3
Quality LevelMQ100
ApplicationDetect mPER1 (residues 6-21) using this Anti-mPER1 (residues 6-21) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesImmunohistichemistry:
Tested on whole brain (SCN; peak staining at CT/ZT12). Optimium working dilutions need to be determined by end used.
Biological Information
ImmunogenRecombinant Protein
SpecificityCat. # AB2201 will recognize the N-terminus of mPER1.
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteMouse, Human and Rat. May react with Drosophila, Arvicanthis niloticus and Mesocricetus auratus based on sequence homology. Reactivity with other species has not been tested.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CAB2
  • KIAA0482
  • MGC88021
  • MGC9753
  • PER
  • PERLD1
  • hPER
  • hPER1
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences CLOCK function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation.
SUBUNIT: Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS, PER2, PER3 and, through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts with GPRASP1 (By similarity).
Q9R1X4:Timeless; NbExp=2; IntAct=EBI-1266764, EBI-1793117;

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
TISSUE SPECIFICITY: In brain, highest expression is observed in the SCN. Highly expressed in the pyramidal cell layer of the piriform cortex, the periventricular part of the caudate-putamen, many thalamic nuclei, and the granular layer of the cerebellar cortex. Weaker expression is detected in most area of the brain, including cortical and non cortical structures. Expression but no oscillations occurs in the glomerular and mitral cell layers of the olfactory bulb, the internal granular layer of the cerebellum, the cornu ammonis and dentate gyrus of the hyppocampus, the cerebral and piriform cortices. Also found in heart, brain, spleen, lung, liver, skeletal muscle, testis. Highest level in testis. Low level in kidney.
DEVELOPMENTAL STAGE: Expressed in the suprachiasmatic nucleus (SCN) during late fetal and early neonatal life.
INDUCTION: In the suprachiasmatic nucleus (SCN), behaves like a day-type oscillator, with maximum expression during the light period. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. In liver, peak levels at CT9. In the SCN, levels increase by light exposure during subjective night. Circadian oscillations also observed in skeletal muscle and liver but not in testis.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation.
PTM: Ubiquitinated.
SIMILARITY: Contains 1 PAC (PAS-associated C-terminal) domain.
SIMILARITY: Contains 2 PAS (PER-ARNT-SIM) domains.
Molecular Weight136 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely tested by western blot on human brain lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Global Trade Item Number
Catalog Number GTIN
AB2201 04053252362163


Anti-mPER1 (residues 6-21) Antibody SDS


Safety Data Sheet (SDS) 

Anti-mPER1 (residues 6-21) Antibody Certificates of Analysis

TitleLot Number
Anti-mPER1 (residues 6-21) - 2424748 2424748
Anti-mPER1 (residues 6-21) - 2042772 2042772
Anti-mPER1 (residues 6-21) - 2204999 2204999
Anti-mPER1 (residues 6-21) - 2227176 2227176
Anti-mPER1 (residues 6-21) - 2296774 2296774
Anti-mPER1 (residues 6-21) - 3255337 3255337
Anti-mPER1 (residues 6-21) - 3480987 3480987
Anti-mPER1 (residues 6-21) - 3953645 3953645
Anti-mPER1 (residues 6-21) - NG1539518 NG1539518
Anti-mPER1 (residues 6-21) -2697509 2697509


Reference overviewApplicationPub Med ID
KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.
Lee, Y; Jang, AR; Francey, LJ; Sehgal, A; Hogenesch, JB
eLife  4  2015

Show Abstract
26319354 26319354
Responses of brain and behavior to changing day-length in the diurnal grass rat (Arvicanthis niloticus).
Leach, G; Ramanathan, C; Langel, J; Yan, L
Neuroscience  234  31-9  2013

Show Abstract
Immunohistochemistry23313227 23313227
Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus.
LeSauter, J; Lambert, CM; Robotham, MR; Model, Z; Silver, R; Weaver, DR
PloS one  7  e35938  2012

Show Abstract
22558277 22558277
Nighttime dim light exposure alters the responses of the circadian system.
D Shuboni,L Yan
Neuroscience  170  2010

Show Abstract
20705120 20705120
Rapid resetting of the mammalian circadian clock.
Best, J D, et al.
J. Neurosci., 19: 828-35 (1999)  1999

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