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07-414 Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)

07-414
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-414
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
      Background InformationArginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).
      References
      Product Information
      FormatSerum
      Control
      • Jurkat cell lysate was probed with Anti-dimethyl-Arginine, asymmetric. Several proteins containing asymmetrically dimethylated arginines were detected, including p110, p75 and Sam68.

        Included Positive Antigen Control:
        Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationRabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C.
      Quality LevelMQ100
      Applications
      ApplicationDetect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Modifications
      • Methylation
      Purification MethodUnpurified
      Molecular WeightVaries. Antibody detects proteins containing asymmetrical dimethylated arginines.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells.

      Western Blot Analysis:
      A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2387832 2387832
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2453240 2453240
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2459628 2459628
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 0704057819 0704057819
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2168338 2168338
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2250514 2250514
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2327463 2327463
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 24346 24346
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 30149 30149
      Anti-dimethyl-Arginine, asymmetric (ASYM24) - 3027216 3027216

      References

      Reference overviewApplicationSpeciesPub Med ID
      LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2.
      Hussein, MA; Shrestha, E; Ouimet, M; Barrett, TJ; Leone, S; Moore, KJ; Hérault, Y; Fisher, EA; Garabedian, MJ
      PloS one  10  e0135218  2015

      Show Abstract
      26288135 26288135
      5-Hydroxymethylcytosine Plays a Critical Role in Glioblastomagenesis by Recruiting the CHTOP-Methylosome Complex.
      Takai, H; Masuda, K; Sato, T; Sakaguchi, Y; Suzuki, T; Suzuki, T; Koyama-Nasu, R; Nasu-Nishimura, Y; Katou, Y; Ogawa, H; Morishita, Y; Kozuka-Hata, H; Oyama, M; Todo, T; Ino, Y; Mukasa, A; Saito, N; Toyoshima, C; Shirahige, K; Akiyama, T
      Cell reports  9  48-60  2014

      Show Abstract
      25284789 25284789
      JMJD6 regulates ERα methylation on arginine.
      Poulard, C; Rambaud, J; Hussein, N; Corbo, L; Le Romancer, M
      PloS one  9  e87982  2014

      Show Abstract
      24498420 24498420
      Myofibrillar Ca(2+) sensitivity is uncoupled from troponin I phosphorylation in hypertrophic obstructive cardiomyopathy due to abnormal troponin T.
      Bayliss, CR; Jacques, AM; Leung, MC; Ward, DG; Redwood, CS; Gallon, CE; Copeland, O; McKenna, WJ; Dos Remedios, C; Marston, SB; Messer, AE
      Cardiovascular research  97  500-8  2013

      Show Abstract
      23097574 23097574
      Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo.
      Scaramuzzino, C; Monaghan, J; Milioto, C; Lanson, NA; Maltare, A; Aggarwal, T; Casci, I; Fackelmayer, FO; Pennuto, M; Pandey, UB
      PloS one  8  e61576  2013

      Show Abstract
      Western Blotting23620769 23620769
      FUS/TLS assembles into stress granules and is a prosurvival factor during hyperosmolar stress.
      Sama, RR; Ward, CL; Kaushansky, LJ; Lemay, N; Ishigaki, S; Urano, F; Bosco, DA
      Journal of cellular physiology  228  2222-31  2013

      Show Abstract
      Immunofluorescence23625794 23625794
      Amyotrophic lateral sclerosis-linked FUS/TLS alters stress granule assembly and dynamics.
      Baron, DM; Kaushansky, LJ; Ward, CL; Sama, RR; Chian, RJ; Boggio, KJ; Quaresma, AJ; Nickerson, JA; Bosco, DA
      Molecular neurodegeneration  8  30  2013

      Show Abstract
      Immunocytochemistry24090136 24090136
      Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.
      Fanis, P; Gillemans, N; Aghajanirefah, A; Pourfarzad, F; Demmers, J; Esteghamat, F; Vadlamudi, RK; Grosveld, F; Philipsen, S; van Dijk, TB
      Molecular & cellular proteomics : MCP  11  1263-73  2012

      Show Abstract
      Western BlottingHuman22872859 22872859
      The effect of PRMT1-mediated arginine methylation on the subcellular localization, stress granules, and detergent-insoluble aggregates of FUS/TLS.
      Yamaguchi, A; Kitajo, K
      PloS one  7  e49267  2012

      Show Abstract
      Immunofluorescence23152885 23152885
      Suppression of PRMT6-mediated arginine methylation of p16 protein potentiates its ability to arrest A549 cell proliferation.
      Xiuli Wang,Ying Huang,Jing Zhao,Yu Zhang,Jun Lu,Baiqu Huang
      The international journal of biochemistry & cell biology  44  2012

      Show Abstract
      23032699 23032699

      Related Products & Applications

      Included Positive Control

      Catalog Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies