OP119 Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90)

OP119
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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OP119-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~185 kDa c-ErbB3/HER3 protein in T47D cells and breast carcinoma tissue.
      Catalogue NumberOP119
      Brand Family Calbiochem®
      SynonymsAnti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
      References
      ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
      Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
      Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
      Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
      Shoyab, M., et al. 1989. Science 243, 1074.
      Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlT-47D cells or breast or prostate carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Not Western Blot
      Not Neutralization Studies
      Flow Cytometry
      Immunofluorescence
      Immunoprecipitation
      Application NotesFlow Cytometry (see comments)
      Immunoblotting (not recommended)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Neutralization Studies (not recommended)
      Application CommentsDoes not cross-react with c-erbB1 (EGFR), c-erbB2/HER2/neu or c-erbB4/HER4. It does not block the binding of heregulin to c-erbB3, has no effect on tyrosine kinase activity, receptor internalization or tumorigenic growth of cells overexpressing c-erbB3. This antibody is particularly well suited for immunoprecipitating the unoccupied as well as the occupied receptor. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human ErbB3 extracellular domain
      ImmunogenHuman
      CloneH3.90.6 or Ab90
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90) Certificates of Analysis

      TitleLot Number
      OP119

      References

      Reference overview
      Chen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
      Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
      Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
      Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
      Shoyab, M., et al. 1989. Science 243, 1074.
      Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-Febuary-2009 RFH
      SynonymsAnti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus
      ApplicationFlow Cytometry (see comments)
      Immunoblotting (not recommended)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Neutralization Studies (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with NSO mouse myeloma cells. Recognizes the ~185 kDa c-ErbB3 protein.
      BackgroundErbB3/HER3, a member of the epidermal growth factor receptor (EGF-R) family, was originally isolated as a cDNA clone from a human carcinoma cell line. The molecule is transcribed from a 6-kilobase mRNA, which can be identified in a variety of different tissues. Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF, amphiregulin, or transforming growth factor-alpha (TGF-α) to the receptor or to other members of the receptor family activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein family. While there is a large degree of conserved homology between the cytoplasmic regions of EGF-R and HER2/neu, the HER3 molecule is more unique. This observation suggests that HER3 is subject to regulatory signals distinct from those affecting EGF-R and HER2/neu and, therefore, signals an alternate biochemical response. In particular, it has been shown that the ligand for HER3 is NDF/heregulin a property shared with HER4. c-ErbB3/HER3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon).
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human ErbB3 extracellular domain
      CloneH3.90.6 or Ab90
      IsotypeIgG2a
      Specieshuman
      Positive controlT-47D cells or breast or prostate carcinoma tissue
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsDoes not cross-react with c-erbB1 (EGFR), c-erbB2/HER2/neu or c-erbB4/HER4. It does not block the binding of heregulin to c-erbB3, has no effect on tyrosine kinase activity, receptor internalization or tumorigenic growth of cells overexpressing c-erbB3. This antibody is particularly well suited for immunoprecipitating the unoccupied as well as the occupied receptor. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChen, X., et al. 1996. J. Biol. Chem. 271, 7620.
      Carraway, K.L., III, et al. 1994. J. Biol. Chem. 269, 14303.
      Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 7792.
      Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 4905.
      Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9193.
      Shoyab, M., et al. 1989. Science 243, 1074.
      Kawakami, T., et al. 1986. Mol. Cell. Biol. 6, 4195.