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OP67 Anti-c-ErbB2/c-Neu (Ab-6) Mouse mAb (OD-3)

OP67
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
OP67-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~97-115 kDa truncated form of c-ErbB2/c-Neu in human serum as well as the ~185 kDa full-length protein. Does not cross-react with the EGF receptor.
Catalogue NumberOP67
Brand Family Calbiochem®
SynonymsAnti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-Human Epidermal Growth Factor, Anti-HER2
References
ReferencesCarney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Bargmann, C.I., et al. 1968. Nature 319, 226.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlHT 1080 or HepG2 cells
Positive controlSK-BR-3 cell conditioned medium
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
Key Applications Immunoblotting (Western Blotting)
Not Frozen Sections
Not Immunofluorescence
Not Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (10 µg/ml)
Frozen Sections (not recommended)
Paraffin Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Application CommentsThis antibody reacts with an extracellular epitope and does not cross react with human EGF-receptor. For best results, use IgM-specific secondary reagents. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogencells overexpressing human c-Neu
ImmunogenHuman
CloneOD-3
HostMouse
IsotypeIgM
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
OP67-100UG 04055977227314

Documentation

Anti-c-ErbB2/c-Neu (Ab-6) Mouse mAb (OD-3) Certificates of Analysis

TitleLot Number
OP67

References

Reference overview
Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Bargmann, C.I., et al. 1968. Nature 319, 226.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
SynonymsAnti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-Human Epidermal Growth Factor, Anti-HER2
ApplicationImmunoblotting (10 µg/ml)
Frozen Sections (not recommended)
Paraffin Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing with SP2/0 mouse myeloma cells. Recognizes the ~185 kDa full-length and the ~97-115 kDa truncated forms of c-ErbB2/c-Neu.
BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
HostMouse
Immunogen speciesHuman
Immunogencells overexpressing human c-Neu
CloneOD-3
IsotypeIgM
Specieshuman, not mouse, not rat
Positive controlSK-BR-3 cell conditioned medium
Negative controlHT 1080 or HepG2 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody reacts with an extracellular epitope and does not cross react with human EGF-receptor. For best results, use IgM-specific secondary reagents. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesCarney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Bargmann, C.I., et al. 1968. Nature 319, 226.
Application referencesImmunoblotting Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.