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CBL202 Anti-Vimentin Antibody, clone VIM 3B4

CBL202
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Am, B, Ca, Ch, H, MkELISA, IF, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL202
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vimentin Antibody, clone VIM 3B4
      References
      Product Information
      FormatPurified
      Control
      • Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
      PresentationProtein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot
      Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
      Immunofluorescence
      ELISA

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenVimentin purified from bovine lens
      CloneVIM 3B4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHighly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
      IsotypeIgG2a
      Species Reactivity
      • Amphibian
      • Bovine
      • Canine
      • Chicken
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlong with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
      Gene Symbol
      • VIM
      • FLJ36605
      • Vimentin.
      • vimentin
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
      SIZE: 466 amino acids; 53652 Da
      SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
      TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
      PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
      SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
      Molecular Weight57 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2–8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Vimentin Antibody, clone VIM 3B4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vimentin Antibody, clone VIM 3B4 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN VIMENTIN 2465136
      MOUSE ANTI-HUMAN VIMENTIN MONOCLONAL ANTIBODY - 2377348 2377348
      MOUSE ANTI-HUMAN VIMENTIN - 3156831 3156831
      MOUSE ANTI-HUMAN VIMENTIN - 3243497 3243497
      MOUSE ANTI-HUMAN VIMENTIN - 3286870 3286870
      MOUSE ANTI-HUMAN VIMENTIN - 3462189 3462189
      MOUSE ANTI-HUMAN VIMENTIN - 3675955 3675955
      MOUSE ANTI-HUMAN VIMENTIN - 3746399 3746399
      MOUSE ANTI-HUMAN VIMENTIN - 3772408 3772408
      MOUSE ANTI-HUMAN VIMENTIN - 3854569 3854569

      Data Sheet

      Title
      MOUSE ANTI-HUMAN VIMENTIN

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      Alternative Packsize

      Catalog Number Description  
      CBL202-K Vimentin Ms x Hu Mg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies