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AB1620 Anti-Vimentin Antibody

AB1620
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MaICC, IHC, IH(P), WBGtSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1620
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vimentin Antibody
      References
      Product Information
      FormatSerum
      Control
      • Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
      PresentationNeat goat Serum. Liquid containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.

      Immunocytochemistry

      Western blot

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenVimentin purified from cultured human foreskin fibroblasts.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGoat
      SpecificityRecognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques.
      Species Reactivity
      • Human
      • Mammals
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlong with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
      Gene Symbol
      • VIM
      • FLJ36605
      • Vimentin.
      • vimentin
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
      SIZE: 466 amino acids; 53652 Da
      SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
      TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
      PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
      SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
      Molecular Weight58 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Vimentin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vimentin Antibody Certificates of Analysis

      TitleLot Number
      GOAT ANTI-HUMAN VIMENTIN 2465120
      GOAT ANTI-HUMAN VIMENTIN POLYCLONAL ANTIBODY - 2446720 2446720
      GOAT ANTI-HUMAN VIMENTIN - 3179947 3179947
      GOAT ANTI-HUMAN VIMENTIN - 3221985 3221985
      GOAT ANTI-HUMAN VIMENTIN - 3257091 3257091
      GOAT ANTI-HUMAN VIMENTIN - 3280417 3280417
      GOAT ANTI-HUMAN VIMENTIN - 3388476 3388476
      GOAT ANTI-HUMAN VIMENTIN - 3475680 3475680
      GOAT ANTI-HUMAN VIMENTIN - 3762608 3762608
      GOAT ANTI-HUMAN VIMENTIN -2528754 2528754

      Data Sheet

      Title
      Anti-Vimentin - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies