Millipore Sigma Vibrant Logo

MAB5504 Anti-Vesicular Glutamate Transporter 2 Antibody

MAB5504
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5504
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vesicular Glutamate Transporter 2 Antibody
      Alternate Names
      • VGLUT2
      Background InformationVesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID: 84487) in rat. VGluT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGLUT1- and VGLUT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGLUT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. Expression of VGLUT2 is reported to increase sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationMouse monoclonal in PBS containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Vesicular Glutamate Transporter 2 using this Anti-Vesicular Glutamate Transporter 2 Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: A 1:50-1:300 dilution of a previous lot worked using ABC with NiDAB on floating mouse brain sections.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant full- length rat Vesicular Glutamate Transporter 2 (VGLUT2)
      Clone8G9.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with VGLUT2 (Vesicular Glutamate Transporter 2).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse and rat. Expected to react with human based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC17A6
      • DNPI
      • VGLUT2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas. May also mediate the transport of inorganic phosphate.
      SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome.
      TISSUE SPECIFICITY: Expressed in brain (at protein level). Expressed in brainstem, deep nuclei, septal nuclei, nuclei of the diagonal band, frontal cortex, hypothalamus, midbrain, parietal cortex and temporal cortex. Also expressed in pineal gland and islets of Langerhans.
      SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

      SIZE: 582 amino acids; 64392 Da
      Molecular Weight56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse brain lysates.

      Western Blotting Analysis: 1:500 dilution of this antibody detected VGLUT2 on 10 µg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Vesicular Glutamate Transporter 2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vesicular Glutamate Transporter 2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Vesicular Glutamate Transporter -2641670 2641670
      Anti-Vesicular Glutamate Transporter -2789802 2789802
      Anti-Vesicular Glutamate Transporter -2817471 2817471
      Anti-Vesicular Glutamate Transporter 2 - 2142176 2142176
      Anti-Vesicular Glutamate Transporter 2 - 2393926 2393926
      Anti-Vesicular Glutamate Transporter 2 - 1976404 1976404
      Anti-Vesicular Glutamate Transporter 2 - 2012363 2012363
      Anti-Vesicular Glutamate Transporter 2 - 2043193 2043193
      Anti-Vesicular Glutamate Transporter 2 - 2090451 2090451
      Anti-Vesicular Glutamate Transporter 2 - 2225163 2225163

      References

      Reference overviewApplicationSpeciesPub Med ID
      Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
      Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
      Nature neuroscience  18  386-92  2015

      Show Abstract
      25664911 25664911
      Interplay between glucose and leptin signalling determines the strength of GABAergic synapses at POMC neurons.
      Lee, DK; Jeong, JH; Chun, SK; Chua, S; Jo, YH
      Nature communications  6  6618  2015

      Show Abstract
      25808323 25808323
      Processing of visually evoked innate fear by a non-canonical thalamic pathway.
      Wei, P; Liu, N; Zhang, Z; Liu, X; Tang, Y; He, X; Wu, B; Zhou, Z; Liu, Y; Li, J; Zhang, Y; Zhou, X; Xu, L; Chen, L; Bi, G; Hu, X; Xu, F; Wang, L
      Nature communications  6  6756  2015

      Show Abstract
      25854147 25854147
      Cerebellar zonal patterning relies on Purkinje cell neurotransmission.
      White, JJ; Arancillo, M; Stay, TL; George-Jones, NA; Levy, SL; Heck, DH; Sillitoe, RV
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  8231-45  2014

      Show Abstract
      24920627 24920627
      Histological features of layers and sublayers in cortical visual areas V1 and V2 of chimpanzees, macaque monkeys, and humans.
      Balaram, P; Young, NA; Kaas, JH
      Eye and brain  2014  5-18  2014

      Show Abstract
      25788835 25788835
      Multiple knockout mouse models reveal lincRNAs are required for life and brain development.
      Sauvageau, M; Goff, LA; Lodato, S; Bonev, B; Groff, AF; Gerhardinger, C; Sanchez-Gomez, DB; Hacisuleyman, E; Li, E; Spence, M; Liapis, SC; Mallard, W; Morse, M; Swerdel, MR; D'Ecclessis, MF; Moore, JC; Lai, V; Gong, G; Yancopoulos, GD; Frendewey, D; Kellis, M; Hart, RP; Valenzuela, DM; Arlotta, P; Rinn, JL
      eLife  2  e01749  2013

      Show Abstract
      24381249 24381249
      Morphological and neurochemical comparisons between pulvinar and V1 projections to V2.
      Marion, R; Li, K; Purushothaman, G; Jiang, Y; Casagrande, VA
      The Journal of comparative neurology  521  813-32  2013

      Show Abstract
      22826174 22826174
      Purkinje cell ataxin-1 modulates climbing fiber synaptic input in developing and adult mouse cerebellum.
      Ebner, BA; Ingram, MA; Barnes, JA; Duvick, LA; Frisch, JL; Clark, HB; Zoghbi, HY; Ebner, TJ; Orr, HT
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  5806-20  2013

      Show Abstract
      23536093 23536093
      Establishment of topographic circuit zones in the cerebellum of scrambler mutant mice.
      Reeber, SL; Loeschel, CA; Franklin, A; Sillitoe, RV
      Frontiers in neural circuits  7  122  2013

      Show Abstract
      23885237 23885237
      Reduction of mutant ataxin-7 expression restores motor function and prevents cerebellar synaptic reorganization in a conditional mouse model of SCA7.
      Furrer, SA; Waldherr, SM; Mohanachandran, MS; Baughn, TD; Nguyen, KT; Sopher, BL; Damian, VA; Garden, GA; La Spada, AR
      Human molecular genetics  22  890-903  2013

      Show Abstract
      23197655 23197655

      Related Products & Applications

      Related Products

      Catalog Number Description  
      MAB5504A4 Anti-Vesicular Glutamate Transporter 2 Antibody, Alexa Fluor® 488 | MAB5504A4 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies