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PC234L Anti-Vasopressin Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PC234L-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes vasopressin in rat hypothalamus.
Catalogue NumberPC234L
Brand Family Calbiochem®
Application Data
Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
References
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat hypothalamus
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunofluorescence
Application NotesFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)
Application CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrat arginine vasopressin
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PC234L-100UL 04055977224689

Documentation

Anti-Vasopressin Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Vasopressin Rabbit pAb Certificates of Analysis

TitleLot Number
PC234L

References

Reference overview
Sudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
ApplicationFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)
Application Data
Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The neuropeptide vasopressin plays important roles in vasoconstriction, antinociception, behavior, electrolyte balance, and coagulation functions. Vasopressin helps regulate the hypothalamic-pituitary-adrenal axis along with the corticotropin-releasing hormone. The immuno-localization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
HostRabbit
Immunogen speciesRat
Immunogenrat arginine vasopressin
IsotypeIgG
Speciesrat
Positive controlRat hypothalamus
FormLyophilized
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.