ST1553 Anti-VCP Mouse mAb (4A8)

ST1553
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1553-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
      Catalogue NumberST1553
      Brand Family Calbiochem®
      SynonymsAnti-Valosin-Containing Protein
      Application Data
      Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHePG2 cells, human placenta tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
      Clone4A8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-VCP Mouse mAb (4A8) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Valosin-Containing Protein
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human VCP by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human VCP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-VCP Mouse mAb (4A8) (Cat. No. ST1553) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
      BackgroundVCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
      HostMouse
      ImmunogenFull-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
      Clone4A8
      IsotypeIgG2a
      Specieshuman
      Positive controlHePG2 cells, human placenta tissue
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review