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05-1312-I-25UG Anti-Ubiquityl-Histone H2B Antibody, clone 56

25 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RChIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-1312-I-25UG
      DescriptionAnti-Ubiquityl-Histone H2B Antibody, clone 56
      Alternate Names
      • Histone H2B type 1-C/E/F/G/I
      • Histone H2B.1 A
      • Histone H2B.a
      • H2B/al
      • Histone H2B.g
      • H2B/g
      • Histone H2B.h
      • H2B/h
      • Histone H2B.k
      • H2B/k
      • Histone H2B.l
      • H2B/l)
      Background InformationHistone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 8339, 8343, 8344, 8346, 8347) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Histone H2B is one of the core components of the nucleosome, the smallest subunit of chromatin. DNA base pairs are wrapped around an octamer of core histone proteins consisting of two each of Histone H2A, H2B, H3 and H34. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression. Histone H2B is ubiquitylated at Lys120 by RAD6 E2 protein in conjunction with RNF20/RNF40 and this ubiquitinylated form is found preferentially associated with the transcribed regions of highly expressed genes.
      Product Information
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Ubiquityl-Histone H2B, clone 56 Antibody, Cat. No. 05-1312-I, is a highly specific mouse monoclonal antibody that targets Ubiquityl-Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in HeLa, NIH3T3, and PC-12 cell lysate.

      Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
      Biological Information
      ImmunogenA branched peptide corresponding to the ubiquitination site on human H2B.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 56 recognizes histone H2B when ubiquitinated on Lys120. It does not recognize H2B in its unmodified state or ubiquitin unconjugated to H2B.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HIST1H2BC
      • H2BFL
      • HIST1H2BE
      • H2BFH
      • HIST1H2BF
      • H2BFG
      • HIST1H2BG
      • H2BFA
      • HIST1
      • H2BI
      • H2BFK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 cell lysate.
      Western Blotting Analysis: 0.25 µg/mL of this antibody detected Ubiquityl-Histone H2B in 10 µg of PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information