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05-1312-I Anti-Ubiquityl-Histone H2B Antibody, clone 56

05-1312-I
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HChIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1312-I
      DescriptionAnti-Ubiquityl-Histone H2B Antibody, clone 56
      Alternate Names
      • Histone H2B type 1-C/E/F/G/I
      • Histone H2B.1 A
      • Histone H2B.a
      • H2B/al
      • Histone H2B.g
      • H2B/g
      • Histone H2B.h
      • H2B/h
      • Histone H2B.k
      • H2B/k
      • Histone H2B.l
      • H2B/l)
      Background InformationHistone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 8339, 8343, 8344, 8346, 8347) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Histone H2B is one of the core components of the nucleosome, the smallest subunit of chromatin. DNA base pairs are wrapped around an octamer of core histone proteins consisting of two each of Histone H2A, H2B, H3 and H34. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression. Histone H2B is ubiquitylated at Lys120 by RAD6 E2 protein in conjunction with RNF20/RNF40 and this ubiquitinylated form is found preferentially associated with the transcribed regions of highly expressed genes.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ubiquityl-Histone H2B, clone 56 Antibody, Cat. No. 05-1312-I, is a highly specific mouse monoclonal antibody that targets Ubiquityl-Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in HeLa, NIH3T3, and PC-12 cell lysate.

      Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
      Biological Information
      ImmunogenA branched peptide corresponding to the ubiquitination site on human H2B.
      Clone56
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 56 recognizes histone H2B when ubiquitinated on Lys120. It does not recognize H2B in its unmodified state or ubiquitin unconjugated to H2B.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H2BC
      • H2BFL
      • HIST1H2BE
      • H2BFH
      • HIST1H2BF
      • H2BFG
      • HIST1H2BG
      • H2BFA
      • HIST1
      • H2BI
      • H2BFK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected Ubiquityl-Histone H2B in 10 µg of PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Ubiquityl-Histone H2B Antibody, clone 56 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ubiquityl-Histone H2B Antibody, clone 56 Certificates of Analysis

      TitleLot Number
      Anti-Ubiquityl-Histone H2B, clone 56 3030533
      Anti-Ubiquityl-Histone H2B, clone 56 - 3156738 3156738
      Anti-Ubiquityl-Histone H2B, clone 56 - 3249527 3249527
      Anti-Ubiquityl-Histone H2B, clone 56 - 3277975 3277975
      Anti-Ubiquityl-Histone H2B, clone 56 - 3484973 3484973
      Anti-Ubiquityl-Histone H2B, clone 56 - 3718349 3718349
      Anti-Ubiquityl-Histone H2B, clone 56 - 3783553 3783553
      Anti-Ubiquityl-Histone H2B, clone 56 - 3827436 3827436
      Anti-Ubiquityl-Histone H2B, clone 56 Monoclonal Antibody 2975809
      Anti-Ubiquityl-Histone H2B, clone 56 Monoclonal Antibody 3067370