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AB10029 Anti-Ubiquityl Histone H2A.X (Lys119) Antibody

AB10029
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB10029
      DescriptionAnti-Ubiquityl Histone H2A.X (Lys119) Antibody
      Alternate Names
      • H2AXK119Ub
      • Histone H2A.X Ubiquityl K119)
      • H2A histone family, member X
      • H2AX histone
      Background InformationH2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.


      References
      Product Information
      FormatSerum
      Control
      • HeLa acid extract
      PresentationUnpurified rabbit polyclonal containing serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.

      Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
      Biological Information
      ImmunogenLinear peptide containing ubiquitylated Lys119 of human Histone H2A.X.
      EpitopeLys119
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Histone H2A.X when ubiquitylated at Lys119.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rat, bovine, and canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2A/X
      • H2A.X
      • H2AX
      • H2a/x
      Modifications
      • Ubiquitylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

      SIZE: 143 amino acids; 15145 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.

      SUBCELLULAR LOCATION: Nucleus.

      DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.

      DOMAIN: The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.

      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).

      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight~ 23 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Ubiquityl Histone H2A.X (Lys119) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ubiquityl Histone H2A.X (Lys119) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Ubiquityl Histone H2A.X (Lys119) - 2459496 2459496
      Anti-Ubiquityl Histone H2A.X (Lys119) - 1990716 1990716
      Anti-Ubiquityl Histone H2A.X (Lys119) - 2109553 2109553
      Anti-Ubiquityl Histone H2A.X (Lys119) - 2159682 2159682
      Anti-Ubiquityl Histone H2A.X (Lys119) - 2320073 2320073
      Anti-Ubiquityl Histone H2A.X (Lys119) - 2530081 2530081
      Anti-Ubiquityl Histone H2A.X (Lys119) - 3738498 3738498
      Anti-Ubiquityl Histone H2A.X (Lys119) - 3864023 3864023
      Anti-Ubiquityl Histone H2A.X (Lys119) - NG1822443 NG1822443
      Anti-Ubiquityl Histone H2A.X (Lys119) - NG1854455 NG1854455

      References

      Reference overviewApplicationSpeciesPub Med ID
      X-inactivation normalizes O-GlcNAc transferase levels and generates an O-GlcNAc-depleted Barr body.
      Olivier-Van Stichelen, S; Hanover, JA
      Frontiers in genetics  5  256  2014

      Show Abstract
      Western BlottingHuman25136351 25136351
      Inhibition of PRC2 histone methyltransferase activity increases TRAIL-mediated apoptosis sensitivity in human colon cancer cells.
      Benoit, YD; Laursen, KB; Witherspoon, MS; Lipkin, SM; Gudas, LJ
      Journal of cellular physiology  228  764-72  2013

      Show Abstract
      Western Blotting23001792 23001792
      (-)-Epigallocatechin-3-gallate and DZNep reduce polycomb protein level via a proteasome-dependent mechanism in skin cancer cells.
      Choudhury, SR; Balasubramanian, S; Chew, YC; Han, B; Marquez, VE; Eckert, RL
      Carcinogenesis  32  1525-32  2011

      Show Abstract
      21798853 21798853

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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