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MAB1098 Anti-Tumor Necrosis Factor-β Antibody, clone 9B9

MAB1098
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1098
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tumor Necrosis Factor-β Antibody, clone 9B9
      Alternate Names
      • TNF-beta
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin (Protein A-agarose chormotography). One bottle of lyophilized preparation contains:200ug mouse IgG, 2 mg potassium phosphate buffer, 2.9 mg sodium chloride, and 20 mg raffinose.Reconstitution with 1 ml sterile, distilled water results in an antibody concentration of 200 ug/ml.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tumor Necrosis Factor-β Antibody, clone 9B9 detects level of Tumor Necrosis Factor-β & has been published & validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot

      Immunocytochemistry

      Immunohistochemistry

      Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman, recombinant tumor necrosis factor-beta (TNF-beta, lymphotoxin) produced in E. Coli.
      Clone9B9
      HostMouse
      SpecificityThis antibody is use for detection, identification and neutralixzation of TNF-b in cell culture and other biological samples. The antibody reacts with human natural and recombinant TNF-b (lymphotoxin). It does not react with human TNF-a. Determination of cross-reactivity to TNF-b from other species has not been done.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
      Gene Symbol
      • LTA
      • OTTHUMP00000037612
      • TNF-beta
      • OTTHUMP00000037613
      • LT-alpha
      • LT
      • TNFB
      • TNFSF1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01374 # Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
      SIZE: 205 amino acids; 22297 Da
      SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits.
      SUBCELLULAR LOCATION: Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated.
      DISEASE: SwissProt: P01374 # Genetic variations in LTA are associated with susceptibility to leprosy type 4 (LPRS4) [MIM:610988]; also known as susceptibility to age-dependent leprosy. Leprosy is a chronic infectious disease of peripheral sensory nerves caused by Mycobacterium leprae. & Genetic variations in LTA are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.
      SIMILARITY: SwissProt: P01374 ## Belongs to the tumor necrosis factor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months.Freeze only once.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-TUMOR NECROSIS FACTOR-#946; MONOCLONAL ANTIBODY - 2136628 2136628
      MOUSE ANTI-TUMOR NECROSIS FACTOR-b MONOCLONAL ANTIBODY - 2108542 2108542
      MOUSE ANTI-TUMOR NECROSIS FACTOR-β - 3501923 3501923
      MOUSE ANTI-TUMOR NECROSIS FACTOR-β MONOCLONAL ANTIBODY -2612290 2612290
      MOUSE ANTI-TUMOR NECROSIS FACTOR-β MONOCLONAL ANTIBODY -2684232 2684232
      MOUSE ANTI-TUMOR NECROSIS FACTOR-β MONOCLONAL ANTIBODY -2723815 2723815

      References

      Reference overviewPub Med ID
      Monocyte-mediated drug-dependent cellular cytotoxicity: effects on different WEHI 164 target cell lines.
      Austgulen, R, et al.
      Cancer Immunol. Immunother., 22: 176-80 (1986)  1986

      Show Abstract
      3731202 3731202
      Human lymphotoxin.
      Aggarwal, B B
      Meth. Enzymol., 116: 441-8 (1985)  1985

      3878923 3878923
      Human tumor necrosis factor.
      Aggarwal, B B and Kohr, W J
      Meth. Enzymol., 116: 448-56 (1985)  1985

      4088089 4088089
      An endotoxin-induced serum factor that causes necrosis of tumors.
      Carswell, E A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 72: 3666-70 (1975)  1975

      Show Abstract
      1103152 1103152