CBL412A5 Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, Po, Pm, B ICC M AlexaFluor®555 Monoclonal Antibody
      Catalogue NumberCBL412A5
      DescriptionAnti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate
      Alternate Names
      • Tubulin beta-3 chain
      • Tubulin beta-4 chain
      • Tubulin beta-III
      Background InformationTubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
      Product Information
      • Rat E18 primary cortex cells
      PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      ApplicationDetect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate validated for use in ICC.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic peptide corresponding to human Tubulin β-III.
      SpecificityThis antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
      Species Reactivity
      • Rat
      • Mouse
      • Pig
      • Primate
      • Bovine
      Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Mouse, Porcine, Primate, and Bovine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010].
      Gene Symbol
      • MC1R
      • TUBB4
      • beta-4
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

      SUBUNIT STRUCTURE: Dimer of alpha and beta chains.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton.

      TISSUE SPECIFICITY: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

      DOMAIN: The highly acidic C-terminal region may bind cations such as calcium.

      PTM: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules Probable.

      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

      INVOLVEMENT IN DISEASE: Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.

      SEQUENCE SIMILARITIES: Belongs to the tubulin family.
      Molecular WeightThe unconjugated parent antibody (Cat. No. MAB1637) has an observed molecular weight at ~55 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in rat E18 primary cortex cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tubulin β-III in rat E18 primary cortex cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate SDS


      Safety Data Sheet (SDS) 

      Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Tubulin -III, clone TU-20, Alexa Fluor#174;555 Conjugate - 21072692107269
      Anti-Tubulin -III, clone TU-20, Alexa Fluor#174;555 Conjugate - 21940922194092
      Anti-Tubulin -III, clone TU-20, Alexa Fluor#174;555 Conjugate - 22088002208800
      Anti-Tubulin -III, clone TU-20, Alexa Fluor#174;555 Conjugate - Q1988594Q1988594
      Anti-Tubulin -III, clone TU-20, Alexa Fluor®555 Conjugate - 22877742287774
      Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate2978318
      Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate2898396
      Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate - 23958002395800
      Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate - 25134262513426
      Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate - 30450463045046

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      Life Science Research > Antibodies and Assays > Primary Antibodies