MAB380 Anti-Tubulin β Antibody, clone 5H1

MAB380
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, R IHC M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB380
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin β Antibody, clone 5H1
      References
      Product Information
      FormatAscites
      PresentationAscites fluid containing sodium azide. Liquid
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tubulin Antibody, β, clone 5H1 is an antibody against Tubulin for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry at 1:300-1:1,000

      Optimal working dilutions must be determined by end user.

      IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380

      This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

      1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

      2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

      3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

      4) 3 x 10 minute washes in TBS.

      5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

      6) 3 x 10 minute washes in TBS.

      7) ABC Elite (1:200 Vector Labs) in TBS.

      8) 2 x 10 minute washes in TBS.

      9) 1 x 10 minute wash in phosphate buffer (no saline).

      10) DAB reaction with 0.06% NiCl added for intensification.

      11) 2 x 10 minute washes in PBS.

      12) 1 x 10 minute wash in phosphate buffer (no saline).
      Biological Information
      ImmunogenRat tubulin.
      Clone5H1
      HostMouse
      SpecificityBeta tubulin
      IsotypeIgM
      Species Reactivity
      • Bovine
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • MC1R
      • beta-4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tubulin β Antibody, clone 5H1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tubulin β Antibody, clone 5H1 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-BETA TUBULIN MONOCLONAL ANTIBODY - 23895212389521
      MOUSE ANTI-BETA TUBULIN -26078972607897
      MOUSE ANTI-BETA TUBULIN MONOCLONAL ANTIBODY - 19836711983671
      MOUSE ANTI-BETA TUBULIN MONOCLONAL ANTIBODY - 21068832106883
      MOUSE ANTI-BETA TUBULIN MONOCLONAL ANTIBODY - 29983682998368

      References

      Reference overviewPub Med ID
      Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.
      Smith, KT; Sardiu, ME; Martin-Brown, SA; Seidel, C; Mushegian, A; Egidy, R; Florens, L; Washburn, MP; Workman, JL
      Molecular & cellular proteomics : MCP  11  1815-28  2012

      Show Abstract
      22984288 22984288
      A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
      Yun-Hong, Y; Chih-Fan, C; Chia-Wei, C; Yen-Chung, C
      Molecular & cellular proteomics : MCP  10  M110.007138  2011

      Show Abstract
      21715321 21715321
      Control of neural cell composition in poly(ethylene glycol) hydrogel culture with soluble factors.
      Mooney, R; Haeger, S; Lawal, R; Mason, M; Shrestha, N; Laperle, A; Bjugstad, K; Mahoney, M
      Tissue engineering. Part A  17  2805-15  2011

      Show Abstract
      21823990 21823990
      Central nervous system delivery of the antipsychotic olanzapine induces hepatic insulin resistance.
      Martins, PJ; Haas, M; Obici, S
      Diabetes  59  2418-25  2010

      Show Abstract
      20682682 20682682
      Activation of platelet-activating factor receptor and pleiotropic effects on tyrosine phospho-EGFR/Src/FAK/paxillin in ovarian cancer.
      Margarita Aponte, Wei Jiang, Montaha Lakkis, Ming-Jiang Li, Dale Edwards, Lina Albitar, Allison Vitonis, Samuel C Mok, Daniel W Cramer, Bin Ye
      Cancer research  68  5839-48  2008

      Show Abstract Full Text Article
      18632638 18632638
      Microtubule-associated protein MAP1A, MAP1B, and MAP2 proteolysis during soluble amyloid beta-peptide-induced neuronal apoptosis. Synergistic involvement of calpain and caspase-3.
      Fifre, A; Sponne, I; Koziel, V; Kriem, B; Yen Potin, FT; Bihain, BE; Olivier, JL; Oster, T; Pillot, T
      The Journal of biological chemistry  281  229-40  2006

      Show Abstract
      16234245 16234245
      Activation of platelet-activating factor receptor-coupled G alpha q leads to stimulation of Src and focal adhesion kinase via two separate pathways in human umbilical vein endothelial cells.
      Deo, DD; Bazan, NG; Hunt, JD
      The Journal of biological chemistry  279  3497-508  2004

      Show Abstract
      14617636 14617636

      Related Products & Applications

      Related Products

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies