MAB3408 Anti-Tubulin Antibody, beta, clone KMX-1

MAB3408
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A, H, M, R ICC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3408
      Replaces04-1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin Antibody, beta, clone KMX-1
      Alternate Names
      • Tubulin beta-III
      • tubulin, beta 3
      • tubulin, beta 4
      Background InformationMicrotubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Cell lysate
      • A431 Cell lysate
      • MCF7 Cell lysate
      PresentationPurified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

      Western blot:
      0.05-0.5 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenTubulin purified from polycephalum myxamoebae
      CloneKMX-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
      IsotypeIgG2b
      Species Reactivity
      • All
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • MC1R
      • beta-4
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western blot:
      1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tubulin Antibody, beta, clone KMX-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tubulin Antibody, beta, clone KMX-1 Certificates of Analysis

      TitleLot Number
      Anti-Tubulin, beta, clone KMX-1 - 24524932452493
      Anti-Tubulin, beta, clone KMX-1 - 24628452462845
      Anti-Tubulin, beta, clone KMX-1 - 20220312022031
      Anti-Tubulin, beta, clone KMX-1 - 20497972049797
      Anti-Tubulin, beta, clone KMX-1 - 20909332090933
      Anti-Tubulin, beta, clone KMX-1 - 21919692191969
      Anti-Tubulin, beta, clone KMX-1 - 22983632298363
      Anti-Tubulin, beta, clone KMX-1 - 32722933272293
      Anti-Tubulin, beta, clone KMX-1 - LV1475931LV1475931
      Anti-Tubulin, beta, clone KMX-1 - LV1584912LV1584912

      References

      Reference overviewApplicationSpeciesPub Med ID
      Zinc finger independent genome-wide binding of Sp2 potentiates recruitment of histone-fold protein Nf-y distinguishing it from Sp1 and Sp3.
      Völkel, S; Stielow, B; Finkernagel, F; Stiewe, T; Nist, A; Suske, G
      PLoS genetics  11  e1005102  2015

      Show Abstract
      25793500 25793500
      Shifts in temperature within the physiologic range modify strand-specific expression of select human microRNAs.
      Potla, R; Singh, IS; Atamas, SP; Hasday, JD
      RNA (New York, N.Y.)  21  1261-73  2015

      Show Abstract
      26018549 26018549
      Type-1 interferons contribute to oxygen glucose deprivation induced neuro-inflammation in BE(2)M17 human neuroblastoma cells.
      Minter, MR; Zhang, M; Ates, RC; Taylor, JM; Crack, PJ
      Journal of neuroinflammation  11  43  2014

      Show Abstract
      24602263 24602263
      The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity.
      Kempf, A; Tews, B; Arzt, ME; Weinmann, O; Obermair, FJ; Pernet, V; Zagrebelsky, M; Delekate, A; Iobbi, C; Zemmar, A; Ristic, Z; Gullo, M; Spies, P; Dodd, D; Gygax, D; Korte, M; Schwab, ME
      PLoS biology  12  e1001763  2014

      Show Abstract
      24453941 24453941
      Comparative analysis of single and combined APP/APLP knockouts reveals reduced spine density in APP-KO mice that is prevented by APPsα expression.
      Weyer, SW; Zagrebelsky, M; Herrmann, U; Hick, M; Ganss, L; Gobbert, J; Gruber, M; Altmann, C; Korte, M; Deller, T; Müller, UC
      Acta neuropathologica communications  2  36  2014

      Show Abstract
      24684730 24684730
      Localization of heat shock proteins in cerebral cortical cultures following induction by celastrol.
      Chow, AM; Tang, DW; Hanif, A; Brown, IR
      Cell stress & chaperones  19  845-51  2014

      Show Abstract
      24700193 24700193
      Nucleosome positioning and histone modifications define relationships between regulatory elements and nearby gene expression in breast epithelial cells.
      Rhie, SK; Hazelett, DJ; Coetzee, SG; Yan, C; Noushmehr, H; Coetzee, GA
      BMC genomics  15  331  2014

      Show Abstract
      Western Blotting24885402 24885402
      Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
      Çolakoğlu, G; Bergstrom-Tyrberg, U; Berglund, EO; Ranscht, B
      Proceedings of the National Academy of Sciences of the United States of America  111  E394-403  2014

      Show Abstract
      24385581 24385581
      Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.
      Khalouei, S; Chow, AM; Brown, IR
      Cell stress & chaperones  19  321-7  2014

      Show Abstract
      Western Blotting24061851 24061851
      Induction of heat shock proteins in cerebral cortical cultures by celastrol.
      Chow, AM; Tang, DW; Hanif, A; Brown, IR
      Cell stress & chaperones  18  155-60  2013

      Show Abstract
      22865541 22865541

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