ST1099 Anti-TORC2 (454-607) Rabbit pAb

ST1099
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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ST1099-50UL
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      Description
      OverviewRecognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.
      Catalogue NumberST1099
      Brand Family Calbiochem®
      SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
      Application Data
      Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
      References
      ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:2000, see comments)
      Immunoprecipitation (1:100, see comments)
      Application CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TORC2 (454-607) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TORC2 (454-607) Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1099

      References

      Reference overview
      Screaton, R.A., et al. 2004. Cell 119, 61.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2007 RFH
      SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
      ApplicationImmunoblotting (1:2000, see comments)
      Immunoprecipitation (1:100, see comments)
      Application Data
      Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.
      BackgroundTORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.