574788 Anti-Syntaxin 4 (2-23) Rabbit pAb

574788
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M, R Rb Polyclonal Antibody

Pricing & Availability

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574788-1SET
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      Description
      OverviewRecognizes the syntaxin 4 protein. Supplied with a control peptide.
      Catalogue Number574788
      Brand Family Calbiochem®
      References
      ReferencesTellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
      Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
      Bennett, M.K., et al. 1993. Cell 74, 863.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:200-1:500, chemiluminescence)
      Application CommentsTo minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [RDRTHELRQGDNISDDEDEVRV(C)] corresponding to amino acids 2-23 of rat syntaxin 4, conjugated to carrier protein
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl deionized H₂O. Following reconstitution, aliquot and freeze (-20°C). Reconstitute control peptide with 100 µl dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Syntaxin 4 (2-23) Rabbit pAb Certificates of Analysis

      TitleLot Number
      574788

      References

      Reference overview
      Tellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
      Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
      Bennett, M.K., et al. 1993. Cell 74, 863.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-August-2008 RFH
      ApplicationImmunoblotting (1:200-1:500, chemiluminescence)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the syntaxin 4 protein. Supplied with a control peptide (immunogen).
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide [RDRTHELRQGDNISDDEDEVRV(C)] corresponding to amino acids 2-23 of rat syntaxin 4, conjugated to carrier protein
      IsotypeIgG
      Speciesmouse, rat
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
      Preservative≤0.1% sodium azide
      CommentsTo minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. For use as a negative control, preincubate 1 µg control peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl deionized H₂O. Following reconstitution, aliquot and freeze (-20°C). Reconstitute control peptide with 100 µl dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesTellam, J.T., et al. 1997. J. Biol. Chem. 272, 6179.
      Mandon, B., et al. 1996. J. Clin. Invest. 98, 906.
      Bennett, M.K., et al. 1993. Cell 74, 863.