Product Information
Applications
Key Applications Immunocytochemistry
Immunoprecipitation
Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesELISA (1:2500-1:10,000)
Frozen Sections (1:5000)
Immunoblotting (1:200, chemiluminescence)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (5 µl/200 µg total protein, see comments)
Application CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified, bovine brain synapsin I
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Synapsin I Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Synapsin I Rabbit pAb Certificates of Analysis

TitleLot Number
574778

References

Reference overview
Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
ApplicationELISA (1:2500-1:10,000)
Frozen Sections (1:5000)
Immunoblotting (1:200, chemiluminescence)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (5 µl/200 µg total protein, see comments)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the synapsin I protein.
BackgroundSynapsin I is a neuron specific protein that serves as an excellent marker for synaptic terminals. Can be used to estimate synaptic density and synaptogenesis.
HostRabbit
Immunogen speciesBovine
Immunogenpurified, bovine brain synapsin I
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlRat brain
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies