574596 Anti-Superoxide Dismutase (Mn Enzyme) Sheep pAb

574596
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ht, H, M, Rb, R Sh Polyclonal Antibody

Pricing & Availability

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574596-1ML
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      Plastic ampoule 1 ml
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      Description
      OverviewRecognizes the ~25 kDa Mn SOD protein in human liver cell lysates. Does not cross-react with Cu/Zn SOD or human serum.
      Catalogue Number574596
      Brand Family Calbiochem®
      SynonymsAnti-SOD
      References
      Product Information
      FormLiquid
      FormulationIn Glycine-buffered saline, 1 mM EDTA, 0.1% ε-aminocaproic acid, 0.01% benzamidine, pH 7.4.
      Positive controlHuman liver cell lysate
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.
      Key Applications Paraffin Sections
      Free-floating Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesFree-floating Sections (1:1000-1:2000)
      Frozen Sections (1:20-1:200)
      Immunoblotting (1:1000)
      Paraffin Sections (1:20-1:200, see application references)
      Application CommentsDoes not exhibit cross-reactivity with SOD (Cu/Zn) by double diffusion. Does not recognize any proteins in human serum. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhighly purified, human liver SOD Mn
      ImmunogenHuman Liver
      HostSheep
      IsotypeIgG
      Species Reactivity
      • Hamster
      • Human
      • Mouse
      • Rabbit
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Superoxide Dismutase (Mn Enzyme) Sheep pAb Certificates of Analysis

      TitleLot Number
      574596
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      SynonymsAnti-SOD
      ApplicationFree-floating Sections (1:1000-1:2000)
      Frozen Sections (1:20-1:200)
      Immunoblotting (1:1000)
      Paraffin Sections (1:20-1:200, see application references)
      DescriptionSheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the ~25 kDa SOD (Mn) protein.
      BackgroundSuperoxide dismutase Mn enzyme (SOD (Mn)) is a homotetrameric metallo-enzyme of aerobic organisms, comprised of subunits of ~25 kDa. Localized primarily in the mitochondria, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O2-) to molecular oxygen (O2) and hydrogen peroxide (H2O2).
      HostSheep
      Immunogen speciesHuman Liver
      Immunogenhighly purified, human liver SOD Mn
      IsotypeIgG
      Specieshamster (weakly), human, mouse, rabbit, rat
      Positive controlHuman liver cell lysate
      FormLiquid
      FormulationIn Glycine-buffered saline, 1 mM EDTA, 0.1% ε-aminocaproic acid, 0.01% benzamidine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not exhibit cross-reactivity with SOD (Cu/Zn) by double diffusion. Does not recognize any proteins in human serum. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      Application referencesParaffin Sections MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.