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AB5206-50UL Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II is an antibody against Sodium Channel for use in IH & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: SCN2A, NAC2, HBSCI, SCN2A2, HBSCII, SCN2A1, Na(v)1.2, HBA, Nav1.2 UniProt Number: Q99250
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AB5206-50UL
50 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5206-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Sodium Channel Antibody, Voltage Gated, Brain Type II
Alternate Names
  • Nav1.2
  • SCN2A
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Sodium Channel Antibody, Voltage Gated, Brain Type II is an antibody against Sodium Channel for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesAll procedures that are going to receive a full-length protein should be performed at 4 degrees C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Immunohistochemistry: 4-15 mg/mL (1:20-1:75)

Western blot: 1 to 2 mg/mL (1:150-1:300) using ECL. Note: The addition of 0.5-2% Triton to the antibody is recommended with this lot.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide of alpha subunit (amino acids 467-485) of rat type II voltage-gated sodium channel (VGSC) (Accession P04775).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes type II alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

SPECIES REACTIVITIES: It is expected that the antibody will also react with human due to sequence similarity (100%). Other species have not been tested.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryVoltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. It is heterogeneously expressed in the brain, and mutations in this gene have been linked to several seizure disorders. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Gene Symbol
  • SCN2A
  • NAC2
  • HBSCI
  • SCN2A2
  • HBSCII
  • SCN2A1
  • Na(v)1.2
  • HBA
  • Nav1.2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q99250 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
SIZE: 2005 amino acids; 227975 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q99250 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis (By similarity).
DISEASE: SwissProt: Q99250 # Defects in SCN2A are a cause of generalized epilepsy with febrile seizures plus (GEFS+) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN2A are a cause of benign familial neonatal- infantile seizures (BFNIS) [MIM:607745]. BFNIS is an autosomal dominant epilepsy syndromes well recognized in the first year of life. It may be associated with paroxysmal dyskinesias in later childhood and can also occur as a non-familial form.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5206-50UL 04053252335860

Documentation

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II SDS

Title

Safety Data Sheet (SDS) 

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II Certificates of Analysis

TitleLot Number
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2454976 2454976
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL - 3299313 3299313
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL - 3937278 3937278
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2061586 2061586
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL, AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2842040

Data Sheet

Title
RABBIT ANTI-BRAIN TYPE II VOLTAGE GATED SODIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY