Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

PC347 Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb

PC347
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PC347-25UG Plastic ampoule 25 μg
Description
OverviewRecognizes the serotonin 3 (5-HT3) receptor in mouse and rat brain tissue.
Catalogue NumberPC347
Brand Family Calbiochem®
Application Data
Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
References
ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
Morales, M., et al. 1996. Brain Res. 731, 199.
Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
Maricq, A.V., et al. 1991. Science 254, 432.
Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
Derkach, V., et al. 1989. Nature 33, 706.
Product Information
FormLiquid
Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
Positive controlMouse or rat brain tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Not Western Blot
Application NotesFrozen Sections (2-5 µg/ml)
Immunoblotting (not recommended)
Application CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PC347-25UG 04053252047077

Documentation

Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb Certificates of Analysis

TitleLot Number
PC347

References

Reference overview
Morales, M., et al. 1998. J. Comp. Neurol. 385, 385.
Morales, M., et al. 1996. Brain Res. 731, 199.
Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
Maricq, A.V., et al. 1991. Science 254, 432.
Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
Derkach, V., et al. 1989. Nature 33, 706.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2016 JSW
ApplicationFrozen Sections (2-5 µg/ml)
Immunoblotting (not recommended)
Application Data
Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the serotonin (5-HT3) receptor.
BackgroundThe monoamine serotonin (5-hydroxytryptamine) mediates a variety of physiological actions by binding to a number of distinct receptors, pharmacologically classified into several major groups which differ in tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the 5-HT3 receptor is a ligand-gated ion channel that mediates a fast excitatory response. This receptor is a member of the superfamily of ligand-gated ion channels related to the nicotinic, GABAA and NMDA receptors. Although the 5-HT3 receptor was initially identified from the neuroblastoma cell line NCB-20, radioligand binding studies and in situ hybridization studies have localized the 5-HT3 receptor to a unique distribution of neurons, supporting possible roles for the receptor in cognition and sensory input. Recent immunohistochemical analysis of the rat brain revealed a broad distribution of 5-HT3 immunoreactivity, primarily including interneurons and motor neurons.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
IsotypeIgG
Speciesmouse, rat
Positive controlMouse or rat brain tissue
FormLiquid
Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
Morales, M., et al. 1996. Brain Res. 731, 199.
Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
Maricq, A.V., et al. 1991. Science 254, 432.
Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
Derkach, V., et al. 1989. Nature 33, 706.