DP02 Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

DP02
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Sv40 Infected Cells M Monoclonal Antibody

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DP02-200UG
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      Glass bottle 200 μg
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      Description
      OverviewRecognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
      Catalogue NumberDP02
      Brand Family Calbiochem®
      References
      ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative control3T3 cells
      Positive controlSV80 or SV-T2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.
      Key Applications Paraffin Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesAffinity Purification (use Cat. No. DP02A)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
      Paraffin Sections (see application references)
      Application CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified SV40 large T-antigen
      EpitopeWithin amino acids 83-128
      ClonePAb416
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Sv40 Infected Cells
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Certificates of Analysis

      TitleLot Number
      DP02

      References

      Reference overview
      DeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.

      Citations

      Title
    • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
    • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-July-2007 JSW
      ApplicationAffinity Purification (use Cat. No. DP02A)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
      Paraffin Sections (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
      BackgroundSV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
      HostMouse
      Immunogenpurified SV40 large T-antigen
      EpitopeWithin amino acids 83-128
      ClonePAb416
      IsotypeIgG2a
      SpeciesSV40 infected cells
      Positive controlSV80 or SV-T2 cells
      Negative control3T3 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Citation
    • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
    • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
    • Application referencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies