Safety Data Sheet (SDS) 

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-November-2010 RFH
Synonyms	Anti-Sequestosome 1
Application	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Data	Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence. Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
Description	Purified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
Background	SQSTM1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.
Host	Mouse
Immunogen species	NA
Immunogen	Full-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
Clone	2C11
Isotype	IgG2a
Species	human, mouse
Positive control	HeLa cells
Form	Liquid
Formulation	In PBS, pH 7.2.
Preservative	None
Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage	-20°C Avoid freeze/thaw
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Regulatory Review
References	Lee, H,K., et al. 2010. Immunity. 32, 227. Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181. Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868. Gal, J., et al. 2009. J. Neurochem. 111 1062. Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265. Jung, H,S., et al. 2008. Cell Metab. 8, 318.