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DR1057 Anti-SQSTM1 Mouse mAb (2C11)

This Anti-SQSTM1 Mouse mAb (2C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of SQSTM1.

Anti-SQSTM1 Mouse mAb (2C11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Sequestosome 1

DR1057
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

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DR1057-100UG
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      Description
      OverviewRecognizes the SQSTM1 protein HeLa cells.
      Catalogue NumberDR1057
      Brand Family Calbiochem®
      SynonymsAnti-Sequestosome 1
      Application Data
      Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
      Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
      Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
      Gal, J., et al. 2009. J. Neurochem. 111 1062.
      Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
      Jung, H,S., et al. 2008. Cell Metab. 8, 318.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
      ImmunogenNA
      Clone2C11
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SQSTM1 Mouse mAb (2C11) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Lee, H,K., et al. 2010. Immunity. 32, 227.
      Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
      Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
      Gal, J., et al. 2009. J. Neurochem. 111 1062.
      Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
      Jung, H,S., et al. 2008. Cell Metab. 8, 318.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Sequestosome 1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
      BackgroundSQSTM1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.
      HostMouse
      Immunogen speciesNA
      ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
      Clone2C11
      IsotypeIgG2a
      Specieshuman, mouse
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
      Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
      Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
      Gal, J., et al. 2009. J. Neurochem. 111 1062.
      Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
      Jung, H,S., et al. 2008. Cell Metab. 8, 318.