Product Information
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
ImmunogenNA
Clone2C11
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-SQSTM1 Mouse mAb (2C11) SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Lee, H,K., et al. 2010. Immunity. 32, 227.
Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
Gal, J., et al. 2009. J. Neurochem. 111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
Jung, H,S., et al. 2008. Cell Metab. 8, 318.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Sequestosome 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
BackgroundSQSTM1 is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals.
HostMouse
Immunogen speciesNA
ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
Clone2C11
IsotypeIgG2a
Specieshuman, mouse
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
Gal, J., et al. 2009. J. Neurochem. 111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
Jung, H,S., et al. 2008. Cell Metab. 8, 318.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies