Product Information
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1-5 µg/ml
Immunocytochemistry (10 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
Clone1H8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-SKP1A Mouse mAb (1H8) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-S-Phase Kinase-Associated Protein 1
ApplicationImmunoblotting (1-5 µg/ml
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa SKP1A protein.
BackgroundThis gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
Clone1H8
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
Negative control293T cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies