PC130 Anti-Rad51 (Ab-1) Rabbit pAb

PC130
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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PC130-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~43 kDa Rad51 protein in ovarian tissue.
      Catalogue NumberPC130
      Brand Family Calbiochem®
      Application Data
      Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
      References
      ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
      Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
      Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Sharan, S.K., et al. 1997. Nature 386, 304.
      Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
      Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
      Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
      Tashiro, S., et al. 1996. Oncogene 12, 2165.
      Ashley, T., et al. 1995. Chromosoma 104, 19.
      Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
      Positive controlA431 cells, HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:2500)
      Immunoprecipitation (40 µl/500µg total protein from lysate)
      Immunocytochemistry (1:500)
      Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
      Biological Information
      Immunogenrecombinant, human Rad51
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Rad51 (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC130

      References

      Reference overview
      Brendel, V., et al. 1997. J. Mol. Evol. 44, 528.
      Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
      Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Sharan, S.K., et al. 1997. Nature 386, 304.
      Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
      Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
      Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
      Tashiro, S., et al. 1996. Oncogene 12, 2165.
      Ashley, T., et al. 1995. Chromosoma 104, 19.
      Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.

      Citations

      Title
      Viera et al. 2009. J. cell Science 122, 2149.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-June-2015 JSW
      ApplicationImmunoblotting (1:2500)
      Immunoprecipitation (40 µl/500µg total protein from lysate)
      Immunocytochemistry (1:500)
      Application Data
      Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

      Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
      DescriptionAffinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
      BackgroundThe bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant, human Rad51
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlA431 cells, HeLa cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
      Preservative≤0.1% sodium azide
      CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
      Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
      Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Sharan, S.K., et al. 1997. Nature 386, 304.
      Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
      Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
      Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
      Tashiro, S., et al. 1996. Oncogene 12, 2165.
      Ashley, T., et al. 1995. Chromosoma 104, 19.
      Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
      CitationViera et al. 2009. J. cell Science 122, 2149.