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DR1015 Anti-RENT1 Goat pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HGtPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
DR1015-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~140 kDa RENT1 protein in HeLa cells.
Catalogue NumberDR1015
Brand Family Calbiochem®
SynonymsAnti-HUPF1, Anti-NORF1
Application Data
Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
References
ReferencesMendell, J.T., et al. 2002. Science 298, 419.
Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
Product Information
FormLiquid
FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000-1:2500)
Application CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
ImmunogenHuman
HostGoat
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
DR1015-100UG 04055977225907

Documentation

Anti-RENT1 Goat pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-RENT1 Goat pAb Certificates of Analysis

TitleLot Number
DR1015

References

Reference overview
Mendell, J.T., et al. 2002. Science 298, 419.
Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
SynonymsAnti-HUPF1, Anti-NORF1
ApplicationImmunoblotting (1:1000-1:2500)
Application Data
Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the ~140 kDa RENT1 protein.
BackgroundEukaryotes have the ability to recognize and degrade transcripts that harbor premature signals for translational termination. Nonsense and frameshift mutations that result in these premature termination codons play a role in many genetic diseases and some cancers. Nonsense-mediated nRNA decay (NMD) is mediated by several factors including rent1/hUpf1, rent2/hUpf2, and hUpf3. When assembled, these proteins form the surveillence complex. Disruption of the mouse Rent1 gene has been reported to completely disrupt NMD and result in embryos that re-absorb shortly after implantation.
HostGoat
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
IsotypeIgG
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
ReferencesMendell, J.T., et al. 2002. Science 298, 419.
Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.