Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE1033 Anti-RBM4 Antibody

View Products on Sigmaaldrich.com
ABE1033
200 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1033
DescriptionAnti-RBM4 Antibody
Alternate Names
  • RNA-binding protein 4
  • Lark homolog
  • hLark
  • RNA-binding motif protein 4
  • RNA-binding motif protein 4a
  • RBM4
Background InformationRBM4 is a widely expressed RNA-binding factor involved in many cellular process, including alternative splicing of pre-mRNA and translation regulation by modulating alternative 5'-splice site and exon selection on target hnRNA. RBM4 plays roles in development, particularly muscle cell development, clock metabolism and cellular stress. In muscle, RBM4 acts as a muscle cell differentiation-promoting factor and activates exon skipping of the PTB pre-mRNA during muscle cell differentiation, and it antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. RBM4 also mediates various mRNA functions important for myocytes, and RBM4 associates with important miRNAs that are important for muscle cell differentiation. RBM4 is also involved in regulation of the biological clock because it is required for the translational activation of PER1 mRNA in response to circadian clock. In cellular stress, RBM4 recruits EIF4A1 to stimulate IRES-dependent translation initiation in response to cellular stress, and RBM4 associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RBM4 Antibody is an antibody against RBM4 Antibody for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected RBM4 in HeLa and A431 cells.
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human RBM4.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RBM4
  • RBM4A
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~42 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected RBM4 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1033 04055977127300