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05-1050X Anti-Phosphotyrosine Antibody, 4G10® Platinum

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05-1050X
30 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, IF, FC, IHC, IP, ICC, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1050X
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, 4G10® Platinum
Background InformationThe development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
References
Product Information
FormatPurified
Control
  • Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
PresentationA proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2bκ) and PY20 (IgG2b) in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Immunocytochemistry
  • ELISA
Biological Information
Immunogen4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein.
Clone4G10
HostMouse
SpecificityTyrosine-phosphorylated proteins from all species
IsotypeIgG2b
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G-Sepharose™ Chromatography
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
Packaging Information
Material Size30 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-1050X 04053252473012

Documentation

Anti-Phosphotyrosine Antibody, 4G10® Platinum SDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphotyrosine Antibody, 4G10® Platinum Certificates of Analysis

TitleLot Number
4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2003344 2003344
4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2243502 2243502
4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1601872 DAM1601872
4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1821235 DAM1821235
4G10#174; Platinum, Anti-Phosphotyrosine - 2075953 2075953
4G10#174; Platinum, Anti-Phosphotyrosine - DAM1462606 DAM1462606
4G10#174; Platinum, Anti-Phosphotyrosine - JBC1862630 JBC1862630
4G10#174; Platinum, Anti-Phosphotyrosine - R0709B0208 R0709B0208
4G10#174; Platinum, Anti-Phosphotyrosine - R0712B0018 R0712B0018
4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 2491203 2491203

References

Reference overviewApplicationPub Med ID
Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
Zoch, A; Mayerl, S; Schulz, A; Greither, T; Frappart, L; Rübsam, J; Heuer, H; Giovannini, M; Morrison, H
PloS one  10  e0129151  2015

Show Abstract
Western Blotting26258444 26258444
Differential requirement for Src family tyrosine kinases in the initiation, progression, and metastasis of prostate cancer.
Gelman, IH; Peresie, J; Eng, KH; Foster, BA
Molecular cancer research : MCR  12  1470-9  2014

Show Abstract
25053806 25053806
Evidence that TMEM67 causes polycystic kidney disease through activation of JNK/ERK-dependent pathways.
Du, E; Li, H; Jin, S; Hu, X; Qiu, M; Han, R
Cell biology international  37  694-702  2013

Show Abstract
23456819 23456819
Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cells.
Liu, S; Zhang, H; Li, M; Hu, D; Li, C; Ge, B; Jin, B; Fan, Z
Cell death and differentiation  20  456-64  2013

Show Abstract
23154388 23154388
The HPV16 E6 oncoprotein causes prolonged receptor protein tyrosine kinase signaling and enhances internalization of phosphorylated receptor species.
Spangle, JM; Munger, K
PLoS pathogens  9  e1003237  2013

Show Abstract
Western Blotting23516367 23516367
Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.
Wu, H; Lu, Y; Shen, C; Patel, N; Gan, L; Xiong, WC; Mei, L
Neuron  75  94-107  2012

Show Abstract
Western Blotting22794264 22794264
Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling.
Myers, MV; Manning, HC; Coffey, RJ; Liebler, DC
Molecular & cellular proteomics : MCP  11  M111.015222  2012

Show Abstract
22147731 22147731
The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor contains an immunoreceptor tyrosine-based inhibitory motif that activates Shp2.
Philpott, N; Bakken, T; Pennell, C; Chen, L; Wu, J; Cannon, M
Journal of virology  85  1140-4  2011

Show Abstract
Western Blotting21047965 21047965
Nifuroxazide inhibits survival of multiple myeloma cells by directly inhibiting STAT3.
Nelson, EA; Walker, SR; Kepich, A; Gashin, LB; Hideshima, T; Ikeda, H; Chauhan, D; Anderson, KC; Frank, DA
Blood  112  5095-102  2008

Show Abstract Full Text Article
18824601 18824601

Data Sheet

Title
4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b)

Related Products & Applications

Alternative Packsize

Catalog Number Description
05-1050 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies