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OP21 Anti-Pan-Ras (Ab-1) Mouse mAb (F132-62)

OP21
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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OP21-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins including both c- and v- Ras in SW480 and Y1 cells and normal skin tissue.
      Catalogue NumberOP21
      Brand Family Calbiochem®
      References
      ReferencesRodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al., 1992 Science 256, 102.
      Bos, J.L., 1988 Mutation Res. 195, 255.
      Furth, M. E., et al., 1987. Oncogene 1, 47.
      Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al.,1983 Cell 34, 581.
      Ellis, R.W., et al., 1981 Nature 292, 506.
      Shih, T.Y., et al., 1980 Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlSw480 or Y1 cells or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ200
      Applications
      Key Applications Not Western Blot
      Paraffin Sections
      Frozen Sections
      Immunofluorescence
      Immunoprecipitation
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (not recommended)
      Immunofluorescence (2 µg/ml)
      Immunoprecipitation (4 µg/sample)
      Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
      Application CommentsRecognizes the p21 translational products of the H-, K- and N-ras human, as well as other mammalian c- and v-ras oncogenes. Recommended for formalin-fixed and paraffin-embedded tissue sections while Anti-Pan-Ras (Ab-2) Mouse mAb (F111-85) (Cat. No. OP22) is recommended for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant p21 Ras
      Epitopewithin the N-terminus
      CloneF132-62
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Pan-Ras (Ab-1) Mouse mAb (F132-62) Certificates of Analysis

      TitleLot Number
      OP21

      References

      Reference overview
      Rodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al., 1992 Science 256, 102.
      Bos, J.L., 1988 Mutation Res. 195, 255.
      Furth, M. E., et al., 1987. Oncogene 1, 47.
      Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al.,1983 Cell 34, 581.
      Ellis, R.W., et al., 1981 Nature 292, 506.
      Shih, T.Y., et al., 1980 Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (not recommended)
      Immunofluorescence (2 µg/ml)
      Immunoprecipitation (4 µg/sample)
      Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa N-, K-, H-, and v-ras proteins.
      BackgroundThe human ras gene family consists of three identified members, H-, K- and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis remains to be determined.
      HostMouse
      Immunogenrecombinant p21 Ras
      Epitopewithin the N-terminus
      CloneF132-62
      IsotypeIgG2b
      Specieshuman, mouse, rat
      Positive controlSw480 or Y1 cells or normal skin tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsRecognizes the p21 translational products of the H-, K- and N-ras human, as well as other mammalian c- and v-ras oncogenes. Recommended for formalin-fixed and paraffin-embedded tissue sections while Anti-Pan-Ras (Ab-2) Mouse mAb (F111-85) (Cat. No. OP22) is recommended for immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRodenhuis, S. et al., 1992 Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al., 1992 Science 256, 102.
      Bos, J.L., 1988 Mutation Res. 195, 255.
      Furth, M. E., et al., 1987. Oncogene 1, 47.
      Kraus, M.H., et al., 1984 Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al., 1983 Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al.,1983 Cell 34, 581.
      Ellis, R.W., et al., 1981 Nature 292, 506.
      Shih, T.Y., et al., 1980 Nature 287, 686.