Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE676 Anti-PHF1 Antibody, clone 12C2.1

Anti-PHF1, clone 12C2.1, Cat. No. MABE676, is a highly specific mouse monoclonal antibody that targets PHF1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PHF1 Antibody, clone 12C2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: PHF1, PCL1 UniProt Number: O43189
View Products on Sigmaaldrich.com
MABE676
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE676
DescriptionAnti-PHF1 Antibody, clone 12C2.1
Alternate Names
  • PHD finger protein 1
  • hPHF1
  • Polycomb-like protein 1
  • hPCl1
Background InformationPHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PHF1, clone 12C2.1, Cat. No. MABE676, is a highly specific mouse monoclonal antibody that targets PHF1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PHF1 in human esophagus and human tonsil tissue.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHF1 in 10 µg of HeLa cell lysate.
Biological Information
ImmunogenGST/His-tagged recombinat fragment corresponding to 137 amino acids from human PHD finger protein 1.
Clone12C2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12C2.1 specifically detects PHF1 in human tissues. It targets an epitope within 137 amino acids from the internal region.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PHF1
  • PCL1
UniProt Number
Molecular Weight~63 kDa observed; 62.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHF1 in 10 µg of mouse heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE676 04054839193576