PC474 Anti-PCNA (247-261) (Ab-5) Rabbit pAb

PC474
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

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PC474-50UL
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      Plastic ampoule 50 ul
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      Description
      OverviewRecognizes the ~37 kDa PCNA protein in MCF-7 cells.
      Catalogue NumberPC474
      Brand Family Calbiochem®
      SynonymsAnti-Proliferating Cell Nuclear Antigen
      Application Data
      Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
      References
      ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
      Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlMCF7 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (1:250-1:1000)
      Application CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PCNA (247-261) (Ab-5) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PCNA (247-261) (Ab-5) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC474

      References

      Reference overview
      Miyachi, K., et al. 1990. J. Immunol. 121, 2228.
      Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-March-2008 JSW
      SynonymsAnti-Proliferating Cell Nuclear Antigen
      ApplicationImmunoprecipitation (1:250-1:1000)
      Application Data
      Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.
      BackgroundThe proliferating cell nuclear antigen (PCNA) is a ~36-37 kDa protein also known as cyclin. The protein has been identified as a polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase PCNA has a very granular distribution and is absent from the nucleloli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cell fixed with aldehydes the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have autoantibodies against PCNA.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
      IsotypeIgG
      Specieshuman
      Positive controlMCF7 cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
      Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.