ST1098 Anti-PAN TORC (1-42) Rabbit pAb

This Anti-PAN TORC (1-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of PAN TORC (1-42).

Anti-PAN TORC (1-42) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TORC3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma translocated protein 1

ST1098
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1098-50UL
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          		 Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~79 kDa TORC1, TORC2, and TORC3 proteins in 293T cells over-expressing TORC1, TORC2, or TORC3. Recognizes endogenous TORC1 and TORC2.
          Catalogue NumberST1098
          Brand Family Calbiochem®
          SynonymsAnti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TORC3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma translocated protein 1
          Application Data
          Detection of human TORC1, TORC2, and TORC3 by immunoblotting. Samples: FLAG-tagged recombinant TORC1 (T1), TORC2 (T2), and TORC3 (T3) immunoprecipitated from over-expressing 293T cells using anti-FLAG antibody. Primary antibody: Anti-PAN-TORC (1-42) Rabbit pAb (Cat. No. ST1098) (1:2000) Detection: chemiluminescence.
          References
          ReferencesConkright, M.D., et al. 2003. Mol. Cell 12, 413.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive control293T cells over-expressing TORC1, TORC2, or TORC3, HepG2, NIH3T3, Min6, and FAO cells
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:2000, see comments)
          Immunoprecipitation (1:100, see comments)
          Application CommentsReadily detects TORC1, TORC2, and TORC3 in cells over-expressing the proteins, as well as endogenous levels of TORC1 and TORC2. Endogenous TORC3 is only detected if present at high levels. This antibody has been used to immunoprecipitate recombinant TORC1, TORC2, and TORC3 from over-expressing cells. For immunoprecipitation 4 µl in a total volume of 400 µl has been tested. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena recombinant protein consisting of amino acids 1-42 of human TORC1 fused to GST
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-PAN TORC (1-42) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-PAN TORC (1-42) Rabbit pAb Certificates of Analysis

          TitleLot Number
          ST1098

          References

          Reference overview
          Conkright, M.D., et al. 2003. Mol. Cell 12, 413.

          Brochure

          Title
          Calbiochem Biologics 32.2
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2007 RFH
          SynonymsAnti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TORC3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma translocated protein 1
          ApplicationImmunoblotting (1:2000, see comments)
          Immunoprecipitation (1:100, see comments)
          Application Data
          Detection of human TORC1, TORC2, and TORC3 by immunoblotting. Samples: FLAG-tagged recombinant TORC1 (T1), TORC2 (T2), and TORC3 (T3) immunoprecipitated from over-expressing 293T cells using anti-FLAG antibody. Primary antibody: Anti-PAN-TORC (1-42) Rabbit pAb (Cat. No. ST1098) (1:2000) Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79 kDa TORC1, TORC2, and TORC3 proteins in over-expressing cells and endogenous TORC1 and TORC2.
          BackgroundTORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
          HostRabbit
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of amino acids 1-42 of human TORC1 fused to GST
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive control293T cells over-expressing TORC1, TORC2, or TORC3, HepG2, NIH3T3, Min6, and FAO cells
          FormLiquid
          FormulationUndiluted serum.
          PreservativeNone
          CommentsReadily detects TORC1, TORC2, and TORC3 in cells over-expressing the proteins, as well as endogenous levels of TORC1 and TORC2. Endogenous TORC3 is only detected if present at high levels. This antibody has been used to immunoprecipitate recombinant TORC1, TORC2, and TORC3 from over-expressing cells. For immunoprecipitation 4 µl in a total volume of 400 µl has been tested. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesConkright, M.D., et al. 2003. Mol. Cell 12, 413.