ST1098 Anti-PAN TORC (1-42) Rabbit pAb

ST1098
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

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ST1098-50UL
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      Description
      OverviewRecognizes the ~79 kDa TORC1, TORC2, and TORC3 proteins in 293T cells over-expressing TORC1, TORC2, or TORC3. Recognizes endogenous TORC1 and TORC2.
      Catalogue NumberST1098
      Brand Family Calbiochem®
      SynonymsAnti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TORC3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma translocated protein 1
      Application Data
      Detection of human TORC1, TORC2, and TORC3 by immunoblotting. Samples: FLAG-tagged recombinant TORC1 (T1), TORC2 (T2), and TORC3 (T3) immunoprecipitated from over-expressing 293T cells using anti-FLAG antibody. Primary antibody: Anti-PAN-TORC (1-42) Rabbit pAb (Cat. No. ST1098) (1:2000) Detection: chemiluminescence.
      References
      ReferencesConkright, M.D., et al. 2003. Mol. Cell 12, 413.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive control293T cells over-expressing TORC1, TORC2, or TORC3, HepG2, NIH3T3, Min6, and FAO cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:2000, see comments)
      Immunoprecipitation (1:100, see comments)
      Application CommentsReadily detects TORC1, TORC2, and TORC3 in cells over-expressing the proteins, as well as endogenous levels of TORC1 and TORC2. Endogenous TORC3 is only detected if present at high levels. This antibody has been used to immunoprecipitate recombinant TORC1, TORC2, and TORC3 from over-expressing cells. For immunoprecipitation 4 µl in a total volume of 400 µl has been tested. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-42 of human TORC1 fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PAN TORC (1-42) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PAN TORC (1-42) Rabbit pAb Certificates of Analysis

      TitleLot Number
      ST1098

      References

      Reference overview
      Conkright, M.D., et al. 2003. Mol. Cell 12, 413.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2007 RFH
      SynonymsAnti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TORC3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma translocated protein 1
      ApplicationImmunoblotting (1:2000, see comments)
      Immunoprecipitation (1:100, see comments)
      Application Data
      Detection of human TORC1, TORC2, and TORC3 by immunoblotting. Samples: FLAG-tagged recombinant TORC1 (T1), TORC2 (T2), and TORC3 (T3) immunoprecipitated from over-expressing 293T cells using anti-FLAG antibody. Primary antibody: Anti-PAN-TORC (1-42) Rabbit pAb (Cat. No. ST1098) (1:2000) Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79 kDa TORC1, TORC2, and TORC3 proteins in over-expressing cells and endogenous TORC1 and TORC2.
      BackgroundTORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-42 of human TORC1 fused to GST
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive control293T cells over-expressing TORC1, TORC2, or TORC3, HepG2, NIH3T3, Min6, and FAO cells
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsReadily detects TORC1, TORC2, and TORC3 in cells over-expressing the proteins, as well as endogenous levels of TORC1 and TORC2. Endogenous TORC3 is only detected if present at high levels. This antibody has been used to immunoprecipitate recombinant TORC1, TORC2, and TORC3 from over-expressing cells. For immunoprecipitation 4 µl in a total volume of 400 µl has been tested. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesConkright, M.D., et al. 2003. Mol. Cell 12, 413.