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506107 Anti-P2X7 Receptor (576-595) Rabbit pAb

506107
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
506107-1SET Glass bottle 1 set
Description
OverviewRecognizes the P2X7 receptor protein. Supplied with a control peptide.
Catalogue Number506107
Brand Family Calbiochem®
Application Data
Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
References
ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
Surprenant, A., et al. 1996. Science 272, 735.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:200-1:300, chemilimunescence)
Application CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
506107-1SET 04055977199437

Documentation

Anti-P2X7 Receptor (576-595) Rabbit pAb Certificates of Analysis

TitleLot Number
506107

References

Reference overview
Collo, G., et al. 1997. Neuropharmacology 36, 1277.
Surprenant, A., et al. 1996. Science 272, 735.

Citations

Title
  • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-August-2007 RFH
    ApplicationImmunoblotting (1:200-1:300, chemilimunescence)
    Application Data
    Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the P2X7 receptor protein. Supplied with control peptide (immunogen).
    HostRabbit
    Immunogen speciesRat
    Immunogena synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
    IsotypeIgG
    Speciesrat
    FormLyophilized
    Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
    Preservative≤0.1% sodium azide (antibody only)
    CommentsThe immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesCollo, G., et al. 1997. Neuropharmacology 36, 1277.
    Surprenant, A., et al. 1996. Science 272, 735.
    Citation
  • Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry 281, 4100-4108.