MABD76 Anti-Oct-4 Antibody, clone 9B7

MABD76
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, IHC, IF M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABD76
      DescriptionAnti-Oct-4 Antibody, clone 9B7
      Alternate Names
      • POU domain, class 5, transcription factor 1
      • Octamer-binding protein 3
      • Oct-3
      • Octamer-binding protein 4
      • Oct-4
      • Octamer-binding transcription factor 3
      • OTF-3
      Background InformationThe POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
      References
      Product Information
      FormatPurified
      Control
      • Mouse embryonic stem cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.

      Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Oct-4.
      Clone9B7
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
      Gene Symbol
      • POU5F1
      • OCT3
      • OCT4
      • OTF3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

      SUBUNIT STRUCTURE: Interacts with UBE2I and ZSCAN10 By similarity. Interacts with PKM2. Interacts with WWP2.

      SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.

      TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
      Molecular Weight~39 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Oct-4 Antibody, clone 9B7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Oct-4 Antibody, clone 9B7 Certificates of Analysis

      TitleLot Number
      Anti-Oct-4, clone 9B7 - 24205442420544
      Anti-Oct-4, clone 9B7 - 20134242013424
      Anti-Oct-4, clone 9B7 - 21611672161167
      Anti-Oct-4, clone 9B7 - 23620002362000
      Anti-Oct-4, clone 9B7 - 32510263251026
      Anti-Oct-4, clone 9B7 - NRG1932577NRG1932577
      Anti-Oct-4, clone 9B7 -25054932505493
      Anti-Oct-4, clone 9B7 -27681122768112
      Anti-Oct-4, clone 9B7 -27846492784649
      Anti-Oct-4, clone 9B7 Monoclonal Antibody2988818

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies