MAB4401C3 Anti-Oct-4 Antibody, clone 10H11.2, Cy3 conjugate

MAB4401C3
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC M Cy3 Monoclonal Antibody
      Description
      Catalogue NumberMAB4401C3
      DescriptionAnti-Oct-4 Antibody, clone 10H11.2, Cy3 conjugate
      Alternate Names
      • POU domain class 5, transcription factor 1
      • Octamer-binding protein 3
      • Oct-3
      • Octamer-binding protein 4
      • Oct-4
      • Octamer-binding transcription factor 3
      • OTF-3
      Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 has been indentified as one of the key genes used to reprogram somatic cells into induced pluripotent stem cells (iPS cells).
      References
      Product Information
      FormatCy3
      Control
      • H9 human embryonic stem cells.
      PresentationPurified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Oct-4 Antibody, clone 10H11.2, Cy3 conjugate, MAB4401C3.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant GST fusion protein, human Oct-4 (aa 1-134).
      Clone10H11.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human Oct-4. Antibody is not reactive with mouse Oct-4 protein.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Antibody is not reactive with mouse Oct-4 protein.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POU5F1
      • Oct-3
      • MGC22487
      • Oct4
      • OTF4
      • OTF3
      • OCT3
      • OCT4
      • Oct-4
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightThe unconjugated parent antibody (Catalog No. MAB4401) has an observed MW of 44 kDA.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Oct-4 in H9 human embryonic stem cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Oct-4 Antibody, clone 10H11.2, Cy3 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Oct-4 Antibody, clone 10H11.2, Cy3 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Oct-4, clone 10H11.2, Cy3 conjugate - Q2044080Q2044080

      References

      Reference overviewPub Med ID
      Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription.
      Wang, Yajuan, et al.
      Biosci. Rep., 31: 109-15 (2011)  2011

      Show Abstract
      20433421 20433421
      The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
      Patricia A Zuk, Patricia A Zuk
      Journal of cellular biochemistry  106  867-77  2009

      Show Abstract
      19199344 19199344
      Targeting cancer stem cells through L1CAM suppresses glioma growth.
      Bao, S; Wu, Q; Li, Z; Sathornsumetee, S; Wang, H; McLendon, RE; Hjelmeland, AB; Rich, JN
      Cancer research  68  6043-8  2008

      Show Abstract
      18676824 18676824

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies