Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||FC, ICC||M||AlexaFluor®488||Monoclonal Antibody|
|Presentation||Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.|
|Immunogen||Recombinant GST fusion protein, human Oct-4 (aa 1-134).|
|Specificity||Reacts with human Oct-4.|
|Antibody Type||Monoclonal Antibody|
|Entrez Gene Number|
|UniProt Summary||FUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
INDUCTION: Transcriptional activity is positively regulated by PKM2.
DOMAIN: The POU-specific domain mediates interaction with PKM2.
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
|Molecular Weight||~44 kDa|
|Safety Information according to GHS|
|Product Usage Statements|
|Quality Assurance||Evaluated by Flow Cytometry in 2102Ep Cells.|
|Storage and Shipping Information|
|Storage Conditions||Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt|
|Material Size||100 tests|
Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate SDS
|Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1741716||NG1741716|
|Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NRG1675588||NRG1675588|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1970353||1970353|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 1990429||1990429|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2063186||2063186|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2110542||2110542|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2196060||2196060|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 2227191||2227191|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1800880||NG1800880|
|Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1832963||NG1832963|
|Reference overview||Pub Med ID|
|Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.|
Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
Nature protocols 7 2029-40 2012
This protocol describes an EDTA-based passaging procedure to be used with chemically defined E8 medium that serves as a tool for basic and translational research into human pluripotent stem cells (PSCs). In this protocol, passaging one six-well or 10-cm plate of cells takes about 6-7 min. This enzyme-free protocol achieves maximum cell survival without enzyme neutralization, centrifugation or drug treatment. It also allows for higher throughput, requires minimal material and limits contamination. Here we describe how to produce a consistent E8 medium for routine maintenance and reprogramming and how to incorporate the EDTA-based passaging procedure into human induced PSC (iPSC) derivation, colony expansion, cryopreservation and teratoma formation. This protocol has been successful in routine cell expansion, and efficient for expanding large-volume cultures or a large number of cells with preferential dissociation of PSCs. Effective for all culture stages, this procedure provides a consistent and universal approach to passaging human PSCs in E8 medium.