FCMAB113A4 Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate

FCMAB113A4
100 tests  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, ICC M AlexaFluor®488 Monoclonal Antibody
      Description
      Catalogue NumberFCMAB113A4
      DescriptionAnti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate
      Alternate Names
      • Octamer-binding transcription factor 3
      • POU class 5 homeobox 1
      • POU domain class 5, transcription factor 1
      • POU domain, class 5, transcription factor 1
      • POU-type homeodomain-containing DNA-binding protein
      • octamer-binding transcription factor-3
      Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Human embryonic stem cells
      PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Flow Cytometry:
      Antibody dilution for cellular staining:
      • Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
      • Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
      • 5 µL for Guava Flow Cytometer
      • 10 µL for other Flow Cytometry instruments
      Applications
      ApplicationDetect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Working Solution Cells / test Total Reaction Volume
      5 μl 5 x 105 Cells / 95 μl PBS 100 μl
      10 μl 1 x 106 Cells / 90 μl PBS 100 μl
      Biological Information
      ImmunogenRecombinant GST fusion protein, human Oct-4 (aa 1-134).
      Clone10H11.2
      HostMouse
      SpecificityReacts with human Oct-4.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC22487
      • OCT3
      • OCT4
      • OTF3
      • OTF4
      • OTTHUMP00000029292
      • Oct-3
      • Oct-4
      • Oct4
      UniProt Number
      UniProt SummaryFUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
      SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
      SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
      TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
      INDUCTION: Transcriptional activity is positively regulated by PKM2.
      DOMAIN: The POU-specific domain mediates interaction with PKM2.
      PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
      Molecular Weight~44 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in 2102Ep Cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor® 488 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1741716NG1741716
      Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NRG1675588NRG1675588
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 19703531970353
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 19904291990429
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 20631862063186
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 21105422110542
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 21960602196060
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - 22271912227191
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1800880NG1800880
      Milli-Mark Anti-Oct-4, clone 10H11.2 Alexa Fluor#174; 488 conjugate - NG1832963NG1832963

      References

      Reference overviewPub Med ID
      Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
      Beers, J; Gulbranson, DR; George, N; Siniscalchi, LI; Jones, J; Thomson, JA; Chen, G
      Nature protocols  7  2029-40  2012

      Show Abstract
      23099485 23099485

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      Categories

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