MAB4401 Anti-Oct-4 Antibody, clone 10H11.2

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, ICC, ELISA M Purified Monoclonal Antibody
      Catalogue NumberMAB4401
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Oct-4 Antibody, clone 10H11.2
      OverviewPurified monoclonal antibody for the detection of Human Oct-4.
      Alternate Names
      • Octamer-binding transcription factor 3
      • POU class 5 homeobox 1
      • POU domain class 5, transcription factor 1
      • POU domain, class 5, transcription factor 1
      • POU-type homeodomain-containing DNA-binding protein
      • octamer-binding transcription factor-3
      Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      Product Information
      • Human embryonic stem cell lysate.
      PresentationPurified Immunoglobulin presented as a liquid in PBS with 0.1% sodium azide.
      Quality LevelMQ100
      ApplicationDetect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      • ELISA
      Application NotesFlow Cytometry: A previous lot of this antibody was used in FC.

      ELISA: A previous lot of this antibody was used in ELISA.

      Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant GST fusion protein, human Oct-4 (aa 1-134).
      Cloneclone 10H11.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      Species Reactivity
      • Human
      Species Reactivity NoteThe clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POU5F1
      • Oct-3
      • MGC22487
      • Oct4
      • OTF4
      • OTF3
      • OCT3
      • OCT4
      • Oct-4
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
      SIZE: 360 amino acids; 38571 Da
      TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
      SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight44 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on mouse P1 brain lysates.

      Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Oct-4 Antibody, clone 10H11.2 SDS


      Safety Data Sheet (SDS) 

      Anti-Oct-4 Antibody, clone 10H11.2 Certificates of Analysis

      TitleLot Number
      Anti-Oct-4, clone 10H11.22474968
      Anti-Oct-4, clone 10H11.2 - 23922842392284
      Anti-Oct-4, clone 10H11.2 - 19912531991253
      Anti-Oct-4, clone 10H11.2 - 20663102066310
      Anti-Oct-4, clone 10H11.2 - 32081783208178
      Anti-Oct-4, clone 10H11.2 - 34073153407315
      Anti-Oct-4, clone 10H11.2 - LV1679970LV1679970
      Anti-Oct-4, clone 10H11.2 - LV1786447LV1786447
      Anti-Oct-4, clone 10H11.2 - NG1865606NG1865606
      Anti-Oct-4, clone 10H11.2 - NG1927045NG1927045


      Reference overviewApplicationPub Med ID
      Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.
      Tadeu, AM; Lin, S; Hou, L; Chung, L; Zhong, M; Zhao, H; Horsley, V
      PloS one  10  e0122493  2015

      Show Abstract
      25849374 25849374
      Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
      Muchkaeva, IA; Dashinimaev, EB; Artyuhov, AS; Myagkova, EP; Vorotelyak, EA; Yegorov, YY; Vishnyakova, KS; Kravchenko, IE; Chumakov, PM; Terskikh, VV; Vasiliev, AV
      Acta naturae  6  45-53  2014

      Show Abstract
      24772326 24772326
      Identification of rabbit annulus fibrosus-derived stem cells.
      Liu, C; Guo, Q; Li, J; Wang, S; Wang, Y; Li, B; Yang, H
      PloS one  9  e108239  2014

      Show Abstract
      25259600 25259600
      Characterization of embryonic stem-like cells derived from HEK293T cells through miR302/367 expression and their potentiality to differentiate into germ-like cells.
      Wang, L; Zhu, H; Wu, J; Li, N; Hua, J
      Cytotechnology  66  729-40  2014

      Show Abstract
      24091881 24091881
      Progerin expression disrupts critical adult stem cell functions involved in tissue repair.
      Pacheco, LM; Gomez, LA; Dias, J; Ziebarth, NM; Howard, GA; Schiller, PC
      Aging  6  1049-63  2014

      Show Abstract
      Western Blotting25567453 25567453
      Metabolic profiling and flux analysis of MEL-2 human embryonic stem cells during exponential growth at physiological and atmospheric oxygen concentrations.
      Turner, J; Quek, LE; Titmarsh, D; Krömer, JO; Kao, LP; Nielsen, L; Wolvetang, E; Cooper-White, J
      PloS one  9  e112757  2014

      Show Abstract
      Western Blotting25412279 25412279
      Synergistic effect of trichostatin A and scriptaid on the development of cloned rabbit embryos.
      Chen, CH; Du, F; Xu, J; Chang, WF; Liu, CC; Su, HY; Lin, TA; Ju, JC; Cheng, WT; Wu, SC; Chen, YE; Sung, LY
      Theriogenology  79  1284-93  2013

      Show Abstract
      23566670 23566670
      High-content imaging-based screening of microenvironment-induced changes to stem cells.
      Vega, SL; Liu, E; Patel, PJ; Kulesa, AB; Carlson, AL; Ma, Y; Becker, ML; Moghe, PV
      Journal of biomolecular screening  17  1151-62  2012

      Show Abstract
      22811477 22811477
      Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
      Chen, CH; Xu, J; Chang, WF; Liu, CC; Su, HY; Chen, YE; Du, F; Sung, LY
      Reproductive biomedicine online  25  358-70  2012

      Show Abstract
      22877942 22877942
      Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
      Chen, CH; Chang, WF; Liu, CC; Su, HY; Shyue, SK; Cheng, WT; Chen, YE; Wu, SC; Du, F; Sung, LY; Xu, J
      Reproductive biomedicine online  24  433-42  2012

      Show Abstract
      22381206 22381206

      Technical Info

      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

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      Alternative Packsize

      Catalog Number Description  
      MAB4401-KC Anti-Oct-4, clone 10H11.2 (1mg) KC Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies