AB3209 Anti-Oct-4 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB3209
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Oct-4 Antibody
      Alternate Names
      • POU5F1
      Background InformationExpression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
      Product Information
      FormatAffinity Purified
      • Pluripotent human embryonic stem (ES) cells
      PresentationAffinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      ApplicationThis Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet.

      Immunohistochemistry: 1:200-1:1,000.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide, amino acids 282-297of Oct4.
      SpecificityRecognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POU5F1
      • Oct-3
      • OTF4
      • OCT3
      • OCT4
      • Oct-4
      • MGC22487
      • Oct4
      • OTF3
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
      SIZE: 360 amino acids; 38571 Da
      TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
      SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight44 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Oct-4 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Oct-4 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-Oct43056061
      RABBIT ANTI-Oct4 - 24921372492137
      RABBIT ANTI-Oct4 - 33803763380376
      RABBIT ANTI-Oct4 -26612832661283
      RABBIT ANTI-Oct4 -26849022684902
      RABBIT ANTI-Oct4 -27806042780604
      RABBIT ANTI-Oct4 -28429852842985


      Reference overviewApplicationPub Med ID
      Method of isolation and characterization of Girardia tigrina stem cells.
      Lopes, KA; DE Campos Velho, NM; Pacheco-Soares, C
      Biomedical reports  3  163-166  2015

      Show Abstract
      25798241 25798241
      Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning.
      Siriboon, C; Lin, YH; Kere, M; Chen, CD; Chen, LR; Chen, CH; Tu, CF; Lo, NW; Ju, JC
      PloS one  10  e0118165  2015

      Show Abstract
      25680105 25680105
      Circulating mouse Flk1+/c-Kit+/CD45- cells function as endothelial progenitors cells (EPCs) and stimulate the growth of human tumor xenografts.
      Russell, JS; Brown, JM
      Molecular cancer  13  177  2014

      Show Abstract
      25047738 25047738
      Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury.
      Yoo, M; Lee, GA; Park, C; Cohen, RI; Schachner, M
      Journal of neurotrauma  31  553-64  2014

      Show Abstract
      24125017 24125017
      Vascular endothelial growth factor receptor 2 (VEGFR-2) plays a key role in vasculogenic mimicry formation, neovascularization and tumor initiation by Glioma stem-like cells.
      Yao, X; Ping, Y; Liu, Y; Chen, K; Yoshimura, T; Liu, M; Gong, W; Chen, C; Niu, Q; Guo, D; Zhang, X; Wang, JM; Bian, X
      PloS one  8  e57188  2013

      Show Abstract
      23536763 23536763
      Variability in the generation of induced pluripotent stem cells: importance for disease modeling.
      Vitale, AM; Matigian, NA; Ravishankar, S; Bellette, B; Wood, SA; Wolvetang, EJ; Mackay-Sim, A
      Stem cells translational medicine  1  641-50  2012

      Show Abstract
      23197870 23197870
      Ciliogenesis in normal human kidney development and post-natal life.
      Saraga-Babić M, Vukojević K, Bočina I, Drnašin K, Saraga M
      Pediatric nephrology (Berlin, Germany)  2011

      Show Abstract
      21688189 21688189
      Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes.
      Jin, J; Jing, W; Lei, XX; Feng, C; Peng, S; Boris-Lawrie, K; Huang, Y
      Nucleic acids research  39  3724-34  2011

      Show Abstract
      21247876 21247876
      Prognostic value of OCT4 in primary intracranial germinoma: a single institute analysis of 31 cases.
      Wanggou S, Jiang X, Yuan X, Ren C, Zeng Y, Li G, Li Q
      British journal of neurosurgery  2011

      Show Abstract
      21973060 21973060
      Foxm1 transcription factor is required for maintenance of pluripotency of P19 embryonal carcinoma cells.
      Xie, Z; Tan, G; Ding, M; Dong, D; Chen, T; Meng, X; Huang, X; Tan, Y
      Nucleic acids research  38  8027-38  2010

      Show Abstract Full Text Article
      20702419 20702419

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      Life Science Research > Antibodies and Assays > Primary Antibodies