OP181 Anti-ΔNp73 Mouse mAb (38C674)

OP181
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
OP181-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~73 kDa ΔNp73 protein.
      Catalogue NumberOP181
      Brand Family Calbiochem®
      Application Data
      Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
      Yang, A., et al. 2000. Nature 404, 99.
      Ueda, Y., et al. 1999. Oncogene 18, 4993.
      Kaghad, M., et al. 1997. Cell 90, 809.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Positive controlCell lines transfected with ΔNp73
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Application CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
      ImmunogenHuman
      Clone38C674
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-ΔNp73 Mouse mAb (38C674) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ΔNp73 Mouse mAb (38C674) Certificates of Analysis

      TitleLot Number
      OP181

      References

      Reference overview
      Levrero, M., et al. 2000 J. Cell Sci. 113, 1661.
      Yang, A., et al. 2000. Nature 404, 99.
      Ueda, Y., et al. 1999. Oncogene 18, 4993.
      Kaghad, M., et al. 1997. Cell 90, 809.

      Citations

      Title
    • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
      Application Data
      Detection of ΔNp73 by immunoblotting. Samples: Whole cell lysates from transfected with ΔNp73 cDNA cell line (lane A), HeLa cells (lane B) and NIH 3T3 cells (lane C) cell lysates. Primary antibody: Anti-ΔNp73 Mouse mAb (38C674) (Cat. No. OP181) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~73 kDa p73 protein lacking the N-terminal transactivation domain, ΔpNp73.
      Backgroundp73 is a relative of tumor suppressor protein p53. It displays a high homology with p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 suggests that they may have overlapping functions involved in the regulation of gene expression. The disruption of p73 leads to hippocampal dysgenesis, hydrocephalus, chronic inflammation, and infections. Splicing variant mRNAs of p73 have been identified in MCF7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxyl-terminal structures, suggesting that this region of p73 may be important for the functions of this protein. Researchers have recently cloned a p73 variant that lacks an amino-terminal transactivation domain, called ΔNp73. The ΔNp73 message is transcribed from an alternative promoter located in intron 3.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
      Clone38C674
      IsotypeIgG₁
      Specieshuman
      Positive controlCell lines transfected with ΔNp73
      FormLiquid
      FormulationIn PBS, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLevrero, M., et al. 2000 J. Cell Sci. 113, 1661.
      Yang, A., et al. 2000. Nature 404, 99.
      Ueda, Y., et al. 1999. Oncogene 18, 4993.
      Kaghad, M., et al. 1997. Cell 90, 809.
      Citation
    • Gemma Dominguez, et al. (2006) Δ-TAp73 Upregulation Correlates With Poor Prognosis in Human Tumors: Putative In Vivo Network Involving p73 Isoforms, p53, and E2F-1. Journal of Clinical Oncology 24, 805-815.