Millipore Sigma Vibrant Logo

ABS90 Anti-Notch 1 Antibody, extracellular

ABS90
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS90
      DescriptionAnti-Notch 1 Antibody, extracellular
      Alternate Names
      • Notch homolog 1, translocation-associated (Drosophila)
      • Notch (Drosophila) homolog 1 (translocation-associated)
      • Translocation-associated notch protein TAN-1
      • neurogenic locus notch homolog protein 1
      Background InformationThe notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human lymph node lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Notch 1 Antibody, extracellular detects level of Notch 1 & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:400 dilution of this antibody has been shown to detect Notch 1 in gastrointestinal and colorectal carcinoma tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the extracellular domain of rat Notch 1.
      EpitopeExtracellular domain
      HostRabbit
      SpecificityThis antibody recognizes Notch 1.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with human and rat. Expected to react with mouse (97% sequence homology), chimpanzee (92% sequence homology) and canine (91% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTransmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD].
      Gene Symbol
      • TAN1
      • notch1
      • hN1
      • Notch 1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity). Acts instructively to control the cell fate determination of CNS multipotent progenitor cells, resulting in astroglial induction and neuron/oligodendrocyte suppression.

      SUBUNIT STRUCTURE: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity).

      Notch 1 intracellular domain: Nucleus (By similarity). Note: Following proteolytical processing NICD is translocated to the nucleus (By similarity).

      TISSUE SPECIFICITY: Expressed in the brain, kidney and spleen. Expressed in postnatal central nervous system (CNS) germinal zones and, in early postnatal life, within numerous cells throughout the CNS. Found in both subventricular and ventricular germinal zones.

      DEVELOPMENTAL STAGE: In the embryo, highest levels occur between days 12 and 14 and decrease rapidly to much lower levels in the adult.

      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).

      Phosphorylated (By similarity).

      O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the NOTCH family.

      Contains 5 ANK repeats.

      Contains 36 EGF-like domains.

      Contains 3 LNR (Lin/Notch) repeats.
      Molecular Weight~260 kDa observed. Notch 1, extracellular is calculated at ~183 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human lymph node lysate.

      Western Blot Analysis: 1:500 dilution of this antibody detected Notch 1 on 10 µg of human lymph node lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Notch 1 Antibody, extracellular SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Notch 1 Antibody, extracellular Certificates of Analysis

      TitleLot Number
      Anti-Notch 1, extracellular - 2420497 2420497
      Anti-Notch 1, extracellular - 2449222 2449222
      Anti-Notch 1, extracellular - 1967280 1967280
      Anti-Notch 1, extracellular - 2293536 2293536
      Anti-Notch 1, extracellular - 2953782 2953782
      Anti-Notch 1, extracellular - 3139144 3139144
      Anti-Notch 1, extracellular - 3718400 3718400
      Anti-Notch 1, extracellular - 3808783 3808783
      Anti-Notch 1, extracellular - NRG1747303 NRG1747303
      Anti-Notch 1, extracellular -2514236 2514236