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07-1232 Anti-Notch 1 Antibody, NT

07-1232
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, WB, IH(P)RbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1232
      Replaces04-1046
      DescriptionAnti-Notch 1 Antibody, NT
      Alternate Names
      • Neurogenic locus notch homolog protein 1 precursor
      • Notch (Drosophila) homolog 1 (translocation-associated)
      • Notch 1
      • Notch homolog 1, translocation-associated (Drosophila)
      • Translocation-associated notch protein TAN-1
      • Neurogenic locus notch homolog protein 1
      Background InformationNotch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
      References
      Product Information
      FormatSerum
      Control
      • Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues.
      PresentationRabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Notch 1 Antibody, N-Terminus is a Rabbit Polyclonal Antibody for detection of Notch 1 also known as Neurogenic locus notch homolog protein 1 precursor & has been validated in ELISA, WB, IHC(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesELISA:
      A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.

      Immunohistochemistry(paraffin):
      Representative testing from a previous lot.
      Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine Cancer

      ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
      Biological Information
      ImmunogenSynthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1.
      EpitopeCleaved N-Terminus
      HostRabbit
      SpecificityNotch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. The antibody is also expected to react with rat due to immunogen sequence homology. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
      Gene Symbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
      SIZE: 2556 amino acids; 272500 Da
      Molecular Weight~80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Notch 1 Antibody, NT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Notch 1 Antibody, NT Certificates of Analysis

      TitleLot Number
      Anti-Notch1, Notch Intracellular -2779490 2779490
      Anti-Notch1, Notch Intracellular Domain (NICD) 2484235
      Anti-Notch1, Notch Intracellular Domain (NICD) 2460205
      Anti-Notch1, Notch Intracellular Domain (NICD) 3068477
      Anti-Notch1, Notch Intracellular Domain (NICD) 3075612
      Anti-Notch1, Notch Intracellular Domain (NICD) - 2384776 2384776
      Anti-Notch1, Notch Intracellular Domain (NICD) - 1966921 1966921
      Anti-Notch1, Notch Intracellular Domain (NICD) - 2005943 2005943
      Anti-Notch1, Notch Intracellular Domain (NICD) - 2036693 2036693
      Anti-Notch1, Notch Intracellular Domain (NICD) - 2069380 2069380

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies