MAB5294-60UL Anti-Neurofilament 70 kDa Antibody, clone DP5 2.7.3

MAB5294-60UL
60 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, Po IHC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5294-60UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament 70 kDa Antibody, clone DP5 2.7.3
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatPurified
      PresentationLiquid. Immunoglobulin purified from ascites fluid.
      Applications
      ApplicationDetect Neurofilament 70 kDa using this Anti-Neurofilament 70 kDa Antibody, clone DP5 2.7.3 validated for use in IH, IH(P).
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry on frozen tissue or paraffin embedded tissue sections: 1:100. The antibody should be diluted in PBS containing 2% BSA, 2% normal horse serum and 0.1% Triton X-100.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenNeurofilament purified from bovine spinal cord.
      EpitopeIdeal for research on xenograft models
      CloneDP5 2.7.3
      HostMouse
      SpecificityNeurofilament 70 kDa. This antibody recognizes neuroblasts, neurons and peripheral nerves from human, bovine and porcine. It DOES NOT recognize neuroblasts, neurons or peripheral nerves from rodent origin (rat and mouse).
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFL
      • NF68
      • CMT2E
      • NFL
      • CMT1F
      • NF-L
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07196 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 543 amino acids; 61517 Da
      DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
      PTM: O-glycosylated (By similarity).
      DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
      SIMILARITY: Belongs to the intermediate filament family.
      MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size60 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament 70 kDa Antibody, clone DP5 2.7.3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament 70 kDa Antibody, clone DP5 2.7.3 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-NEUROFILAMENT 70 HUMAN, BOVINE, PORCINE SPECIFIC MONOCLONAL ANTIBODY - 23283212328321

      References

      Reference overviewApplicationSpeciesPub Med ID
      Neural progenitors from human embryonic stem cells.
      Reubinoff, B E, et al.
      Nat. Biotechnol., 19: 1134-40 (2001)  2001

      Immunohistochemistry (Tissue)Human Only11731782 11731782
      Long-term survival of fetal porcine lateral ganglionic eminence cells in the hippocampus of rats.
      Jacoby, D B, et al.
      J. Neurosci. Res., 56: 581-94 (1999)  1999

      Show Abstract
      10374813 10374813

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies