MAB1615 Anti-Neurofilament 70 kDa Antibody, clone DA2

MAB1615
300 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R WB, ICC, IHC M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMAB1615
      Replaces04-1112
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament 70 kDa Antibody, clone DA2
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatCulture Supernatant
      PresentationLiquid. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Neurofilament 70 kDa Antibody, clone DA2 is validated for use in WB, IC, IH for the detection of Neurofilament 70 kDa.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoblot and immunohistochemistry (frozen sections, little reactivity on formalin-fixed paraffin embedded material). Optimal working dilutions must be determined by end user.

      IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB1615

      This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

      1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

      2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

      3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

      4) 3 x 10 minute washes in TBS.

      5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

      6) 3 x 10 minute washes in TBS.

      7) ABC Elite (1:200 Vector Labs) in TBS.

      8) 2 x 10 minute washes in TBS.

      9) 1 x 10 minute wash in phosphate buffer (no saline).

      10) DAB reaction with 0.06% NiCl added for intensification.

      11) 2 x 10 minute washes in PBS.

      12) 1 x 10 minute wash in phosphate buffer (no saline).
      Biological Information
      ImmunogenEnzymatically dephosphorylated pig neurofilaments.
      CloneDA2
      HostMouse
      SpecificityRecognizes the low molecular weight (68-70 kDa) subunit of the neurofilament triplet (NF-L), the epitope is phosphate independent. Reacts with human and higher vertebrates.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFL
      • CMT1F
      • NF-L
      • NF68
      • CMT2E
      • NFL
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07196 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 543 amino acids; 61517 Da
      DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
      PTM: O-glycosylated (By similarity).
      DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
      SIMILARITY: Belongs to the intermediate filament family.
      MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size300 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament 70 kDa Antibody, clone DA2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament 70 kDa Antibody, clone DA2 Certificates of Analysis

      TitleLot Number
      Anti-Neurofilament 70 kDa, clone DA23072349
      Anti-Neurofilament 70 kDa, clone DA2 - 21459272145927
      Anti-Neurofilament 70 kDa, clone DA2 - 24246622424662
      Anti-Neurofilament 70 kDa, clone DA2 - 20431772043177
      Anti-Neurofilament 70 kDa, clone DA2 - 23325002332500
      Anti-Neurofilament 70 kDa, clone DA2 - 32091843209184
      Anti-Neurofilament 70 kDa, clone DA2 - 32283763228376
      Anti-Neurofilament 70 kDa, clone DA2 - 32602343260234
      Anti-Neurofilament 70 kDa, clone DA2 - JBC1766817JBC1766817
      Anti-Neurofilament 70 kDa, clone DA2 - NG1853886NG1853886

      References

      Reference overviewApplicationPub Med ID
      Primed pluripotent cell lines derived from various embryonic origins and somatic cells in pig.
      Park, JK; Kim, HS; Uh, KJ; Choi, KH; Kim, HM; Lee, T; Yang, BC; Kim, HJ; Ka, HH; Kim, H; Lee, CK
      PloS one  8  e52481  2013

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      23326334 23326334
      TrkB signaling directs the incorporation of newly generated periglomerular cells in the adult olfactory bulb.
      Bergami, M; Vignoli, B; Motori, E; Pifferi, S; Zuccaro, E; Menini, A; Canossa, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  11464-78  2013

      Show Abstract
      23843518 23843518
      KSRP modulation of GAP-43 mRNA stability restricts axonal outgrowth in embryonic hippocampal neurons.
      Bird, CW; Gardiner, AS; Bolognani, F; Tanner, DC; Chen, CY; Lin, WJ; Yoo, S; Twiss, JL; Perrone-Bizzozero, N
      PloS one  8  e79255  2013

      Show Abstract
      24244461 24244461
      A ROCK Inhibitor Blocks the Inhibitory Effect of Chondroitin Sulfate Proteoglycan on Morphological Changes of Mesenchymal Stromal/Stem Cells into Neuron-Like Cells.
      Lim, HS; Joe, YA
      Biomolecules & therapeutics  21  447-53  2013

      Show Abstract
      24404335 24404335
      Investigation of cytoskeleton proteins in neurons of the cat lateral geniculate nucleus.
      Duffy KR, Crowder NA, Ledue EE
      The Journal of comparative neurology  520  186-99. doi  2012

      Show Abstract
      21800310 21800310
      Brilliant violet fluorophores: a new class of ultrabright fluorescent compounds for immunofluorescence experiments.
      Pratip K Chattopadhyay,Brent Gaylord,Adrian Palmer,Nan Jiang,Mary A Raven,Geoff Lewis,Morgan A Reuter,A K M Nur-Ur Rahman,David A Price,Michael R Betts,Mario Roederer
      Cytometry. Part A : the journal of the International Society for Analytical Cytology  81  2012

      Show Abstract
      22489009 22489009
      Beneficial effects of exendin-4 on experimental polyneuropathy in diabetic mice.
      Himeno, T; Kamiya, H; Naruse, K; Harada, N; Ozaki, N; Seino, Y; Shibata, T; Kondo, M; Kato, J; Okawa, T; Fukami, A; Hamada, Y; Inagaki, N; Seino, Y; Drucker, DJ; Oiso, Y; Nakamura, J
      Diabetes  60  2397-406  2011

      Show Abstract
      21810596 21810596
      Differentiation of induced pluripotent stem cells into functional oligodendrocytes.
      Czepiel M, Balasubramaniyan V, Schaafsma W, Stancic M, Mikkers H, Huisman C, Boddeke E, Copray S
      Glia  59  882-92  2011

      Show Abstract
      21438010 21438010
      Cytoarchitecture of the mouse neocortex revealed by the low-molecular-weight neurofilament protein subunit.
      Melissa Paulussen,Sandy Jacobs,Estelle Van der Gucht,Patrick R Hof,Lutgarde Arckens
      Brain structure & function  216  2011

      Show Abstract
      21465412 21465412
      Monocular deprivation provokes alteration of the neuronal cytoskeleton in developing cat lateral geniculate nucleus.
      Kevin R Duffy, Joanna E Slusar, Kevin R Duffy, Joanna E Slusar
      Visual neuroscience  26  319-28  2009

      Show Abstract
      19519963 19519963

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

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      Life Science Research > Antibodies and Assays > Primary Antibodies