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MAB5326 Anti-Nestin Antibody, clone 10C2

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MAB5326
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IHC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5326
ReplacesAB5922
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nestin Antibody, clone 10C2
Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationThis Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.

WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.

Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton

Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenFusion protein.
Epitopehuman
Clone10C2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Gene Symbol
  • NES
  • ORFNames=Nbla00170
  • FLJ21841
  • Nestin.
  • Nbla00170
Non-Reactive Species
  • Mouse
  • Rat
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
SIZE: 1621 amino acids; 177439 Da
SUBUNIT: Interacts with FHOD3 (By similarity).
TISSUE SPECIFICITY: CNS stem cells.
DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
Stem Cell Type
  • Neural Stem Cells
  • Epithelial Cells
Molecular Weight200-220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAntibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB5326 08436037124167

Documentation

Anti-Nestin Antibody, clone 10C2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Nestin Antibody, clone 10C2 Certificates of Analysis

TitleLot Number
Anti-Nestin, clone 10C2 - 2140043 2140043
Anti-Nestin, clone 10C2 - 2393925 2393925
Anti-Nestin, clone 10C2 - 1993773 1993773
Anti-Nestin, clone 10C2 - 2044293 2044293
Anti-Nestin, clone 10C2 - 2110498 2110498
Anti-Nestin, clone 10C2 - 2216871 2216871
Anti-Nestin, clone 10C2 - 2276347 2276347
Anti-Nestin, clone 10C2 - 2325122 2325122
Anti-Nestin, clone 10C2 - 3189195 3189195
Anti-Nestin, clone 10C2 - 3209141 3209141

References

Reference overviewApplicationSpeciesPub Med ID
Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood.
Zhou, H; Martinez, H; Sun, B; Li, A; Zimmer, M; Katsanis, N; Davis, EE; Kurtzberg, J; Lipnick, S; Noggle, S; Rao, M; Chang, S
Stem cell reviews  11  652-65  2015

Show Abstract
25951995 25951995
Early gestation chorionic villi-derived stromal cells for fetal tissue engineering.
Lankford, L; Selby, T; Becker, J; Ryzhuk, V; Long, C; Farmer, D; Wang, A
World journal of stem cells  7  195-207  2015

Show Abstract
25621120 25621120
The changes of angiogenesis and immune cell infiltration in the intra- and peri-tumoral melanoma microenvironment.
Zidlik, V; Brychtova, S; Uvirova, M; Ziak, D; Dvorackova, J
International journal of molecular sciences  16  7876-89  2015

Show Abstract
25913374 25913374
Induced pluripotent stem cell-derived neuronal cells from a sporadic Alzheimer's disease donor as a model for investigating AD-associated gene regulatory networks.
Hossini, AM; Megges, M; Prigione, A; Lichtner, B; Toliat, MR; Wruck, W; Schröter, F; Nuernberg, P; Kroll, H; Makrantonaki, E; Zouboulis, CC; Zoubouliss, CC; Adjaye, J
BMC genomics  16  84  2015

Show Abstract
25765079 25765079
Inhibitor of DNA Binding 4 (ID4) is highly expressed in human melanoma tissues and may function to restrict normal differentiation of melanoma cells.
Peretz, Y; Wu, H; Patel, S; Bellacosa, A; Katz, RA
PloS one  10  e0116839  2015

Show Abstract
25642713 25642713
Bevacizumab treatment induces metabolic adaptation toward anaerobic metabolism in glioblastomas.
Fack, F; Espedal, H; Keunen, O; Golebiewska, A; Obad, N; Harter, PN; Mittelbronn, M; Bähr, O; Weyerbrock, A; Stuhr, L; Miletic, H; Sakariassen, PØ; Stieber, D; Rygh, CB; Lund-Johansen, M; Zheng, L; Gottlieb, E; Niclou, SP; Bjerkvig, R
Acta neuropathologica  129  115-31  2015

Show Abstract
25322816 25322816
Inhibition of Excessive Monoamine Oxidase A/B Activity Protects Against Stress-induced Neuronal Death in Huntington Disease.
Ooi, J; Hayden, MR; Pouladi, MA
Molecular neurobiology  52  1850-61  2015

Show Abstract
25398695 25398695
17β-estradiol protects human eyelid-derived adipose stem cells against cytotoxicity and increases transplanted cell survival in spinal cord injury.
Zhou, J; Lu, P; Ren, H; Zheng, Z; Ji, J; Liu, H; Jiang, F; Ling, S; Heng, BC; Hu, X; Ouyang, H
Journal of cellular and molecular medicine  18  326-43  2014

Show Abstract
ImmunocytochemistryHuman24373095 24373095
Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin.
Mallon, BS; Hamilton, RS; Kozhich, OA; Johnson, KR; Fann, YC; Rao, MS; Robey, PG
Stem cell research  12  376-86  2014

Show Abstract
24374290 24374290
Neonatal immune-tolerance in mice does not prevent xenograft rejection.
Mattis, VB; Wakeman, DR; Tom, C; Dodiya, HB; Yeung, SY; Tran, AH; Bernau, K; Ornelas, L; Sahabian, A; Reidling, J; Sareen, D; Thompson, LM; Kordower, JH; Svendsen, CN
Experimental neurology  254  90-8  2014

Show Abstract
24440640 24440640

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Alternative Packsize

Catalog Number Description
MAB5326-KC Anti-Nestin, clone 10C2 (1mg) KC

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies