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AB5320 Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

AB5320
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5320
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody
      Alternate Names
      • Chondroitin sulfate proteoglycan NG2
      • HSN tumor-specific antigen
      Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat B49 glial cell extracts
      PresentationPurified rabbit polyclonal in PBS buffer containing 0.02% azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NG2 Chondroitin Sulfate Proteoglycan, Cat. No. AB5320, is a rabbit polyclonal antibody that detects rat NG2 Chondroitin Sulfate and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1:250 dilution of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in Rat brain hippocampus tissue sections. Care should be taken to avoid overfixing tissue sections.

      Optimal working dilutions and protocols must be determined by end user
      Biological Information
      ImmunogenRecombinant rat Chondroitin sulfate proteoglycan 4 containing amino acids 30 to 2225.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      Purification MethodAntibody purified by protein A, then placed over a fibronectin affinity column.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
      SIZE: 2322 amino acids; 250497 Da
      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells
      Molecular Weight270-300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates

      Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody Certificates of Analysis

      TitleLot Number
      Anti-NG2 Chondroitin Sulfate -2664415 2664415
      Anti-NG2 Chondroitin Sulfate -2768460 2768460
      Anti-NG2 Chondroitin Sulfate Proteoglycan 3086653
      Anti-NG2 Chondroitin Sulfate Proteoglycan 3166062
      Anti-NG2 Chondroitin Sulfate Proteoglycan 3162426
      Anti-NG2 Chondroitin Sulfate Proteoglycan 2474971
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2115992 2115992
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2148254 2148254
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2328335 2328335
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 2388848 2388848

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure

      Data Sheet

      Title
      RABBIT ANTI-NG2 CHONDROITIN SULFATE PROTEOGLYCAN POLYCLONAL ANTIBODY

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies