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MABD24 Anti-NANOG Antibody, clone 7F7.1

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Catalogue NumberMABD24
      DescriptionAnti-NANOG Antibody, clone 7F7.1
      Alternate Names
      • Homeobox protein NANOG
      • Homeobox transcription factor Nanog
      • hNanog
      Background InformationNANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed.  NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.
      Product Information
      • H9 human embryonic stem cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human NANOG.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NANOG
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).

      SIZE: 305 amino acids; 34620 Da

      SUBUNIT: Interacts with SMAD1 and SALL4 (By similarity).


      TISSUE SPECIFICITY: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.DEVELOPMENTAL STAGE: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2- 16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

      SIMILARITY: Belongs to the Nanog homeobox family. & Contains 1 homeobox DNA-binding domain.

      MISCELLANEOUS: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.
      Molecular Weight~39 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in H9 human embryonic stem cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-NANOG Antibody, clone 7F7.1 SDS


      Safety Data Sheet (SDS) 

      Anti-NANOG Antibody, clone 7F7.1 Certificates of Analysis

      TitleLot Number
      Anti-NANOG, clone 7F7.1 3077371
      Anti-NANOG, clone 7F7.1 2465297
      Anti-NANOG, clone 7F7.1 - 2379504 2379504
      Anti-NANOG, clone 7F7.1 - 2437724 2437724
      Anti-NANOG, clone 7F7.1 - 2030394 2030394
      Anti-NANOG, clone 7F7.1 - 2189736 2189736
      Anti-NANOG, clone 7F7.1 - 2535319 2535319
      Anti-NANOG, clone 7F7.1 - 3324995 3324995
      Anti-NANOG, clone 7F7.1 - 3526393 3526393
      Anti-NANOG, clone 7F7.1 - 3675949 3675949


      Reference overviewPub Med ID
      Glycosyltransferase ST6GAL1 contributes to the regulation of pluripotency in human pluripotent stem cells.
      Wang, YC; Stein, JW; Lynch, CL; Tran, HT; Lee, CY; Coleman, R; Hatch, A; Antontsev, VG; Chy, HS; O'Brien, CM; Murthy, SK; Laslett, AL; Peterson, SE; Loring, JF
      Scientific reports  5  13317  2015

      Show Abstract
      26304831 26304831

      Technical Info

      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

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      Life Science Research > Antibodies and Assays > Primary Antibodies